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Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

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Related Experiment Video

Updated: Jun 10, 2026

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
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Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

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ICAM-5: A Novel Marker for Neuronally Derived Extracellular Vesicles.

Piper Deleon1,2, Reagan Hattenberger1, Barbara Knollmann-Ritschel1

  • 1Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD.

Biorxiv : the Preprint Server for Biology
|November 26, 2025
PubMed
Summary

Researchers identified ICAM-5 as a novel marker for isolating neuronal-derived extracellular vesicles (NDEVs). This specific marker enhances NDEV isolation from biofluids, improving neurological condition diagnosis and monitoring.

Keywords:
BiofluidsBiomarkerExtracellular vesicles

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Biotechnology

Background:

  • Current methods for isolating neuronal-derived extracellular vesicles (NDEVs) from human biofluids lack specificity.
  • Some reported NDEV markers are soluble proteins, not extracellular vesicle (EV)-associated proteins.

Purpose of the Study:

  • Identify a novel, NDEV-associated, and central nervous system-specific marker for NDEV isolation.
  • Validate the marker's efficacy in human serum and cerebrospinal fluid (CSF).

Main Methods:

  • Isolated EVs from human cortical neurons in vitro.
  • Used mass spectrometry to screen for potential EV surface markers.
  • Validated ICAM-5 in human serum and CSF samples, assessing NDEV purity and presence of neurological injury markers.

Main Results:

  • Identified 63 brain-specific proteins, selecting ICAM-5 for further validation.
  • Confirmed ICAM-5 is present on NDEVs, co-localizing with CD-63 and CD-9.
  • Isolated NDEVs using ICAM-5 contained neuronal proteins (tau, NSE) but not glial markers.
  • Detected elevated UCH-L1 levels in TBI patient serum using ICAM-5-isolated EVs compared to other markers.

Conclusions:

  • ICAM-5 is a specific EV-associated marker for isolating NDEVs from human biofluids.
  • ICAM-5 can potentially improve NDEV enrichment from biofluids.
  • This approach may enhance the diagnosis and monitoring of neurological conditions.