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    Area of Science:

    • Biomedical Optics
    • Medical Imaging Technology
    • Ophthalmology

    Background:

    • Polarization-sensitive optical coherence tomography (PS-OCT) quantifies tissue birefringence by analyzing backscattered light polarization.
    • Conventional PS-OCT systems require specialized hardware, limiting their widespread adoption in research and clinical settings.

    Purpose of the Study:

    • To develop a modular PS-OCT framework compatible with standard spectral-domain OCT platforms.
    • To enable quantitative birefringence imaging with minimal hardware modifications for broader accessibility.

    Main Methods:

    • Integration of a detachable rotating achromatic half-wave plate into the sample arm of a commercial OCT system.
    • Acquisition of multiple measurements at different waveplate orientations to reconstruct the sample's round-trip Jones matrix.
    • Implementation of a retarder-constrained phase optimization strategy to correct for phase variations between measurements.

    Main Results:

    • Successful validation of the PS-OCT framework using birefringent phantoms and in vivo human retina imaging.
    • Reliable reconstruction of key polarization properties, including retardance and optic axis orientation.
    • Demonstration of compatibility with mainstream OCT systems, requiring only minor hardware adjustments.

    Conclusions:

    • The developed modular PS-OCT framework significantly lowers technical barriers for implementing advanced birefringence imaging.
    • This approach facilitates the rapid and widespread deployment of PS-OCT across diverse biomedical research and clinical applications.
    • The system's adaptability promotes broader utilization of PS-OCT for enhanced diagnostic capabilities.