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Related Experiment Video

Updated: Jan 10, 2026

Decellularization and Recellularization of Whole Livers
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Development of Acellular Hepatic Scaffolds Through a Low-Cost Gravity-Assisted Perfusion Decellularization Method.

María Fernanda Duarte-Ortega1, Luis Bernardo Enríquez-Sánchez1, Manuel David Pérez-Ruiz1

  • 1Facultad de Medicina y Ciencias Biomédicas, Universidad Autónoma de Chihuahua, Chihuahua 31109, Mexico.

Biomimetics (Basel, Switzerland)
|November 26, 2025
PubMed
Summary

A new gravity-assisted perfusion method efficiently decellularizes rat livers, creating high-quality scaffolds without pumps. This low-cost technique is ideal for tissue engineering and regenerative medicine in resource-limited settings.

Keywords:
acellular scaffoldsbiomimeticsperfusion decellularizationrat livertissue engineering

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Developing cost-effective decellularization methods is crucial for tissue engineering and regenerative medicine.
  • Limited access to specialized perfusion systems hinders progress in resource-constrained regions.

Purpose of the Study:

  • To standardize a gravity-assisted perfusion protocol for rat liver decellularization.
  • To evaluate its efficacy compared to conventional immersion methods.

Main Methods:

  • A gravity-driven vascular flushing method was used for adult Wistar rat liver decellularization.
  • The protocol operated without pumps or pressurized equipment.
  • Scaffolds were assessed via macroscopic inspection, Masson's trichrome staining, and residual DNA quantification.

Main Results:

  • Gravity-assisted perfusion significantly improved cellular removal and extracellular matrix (ECM) preservation over immersion.
  • Residual DNA levels were significantly lower (3.7 ng/mg) in perfused scaffolds, meeting decellularization thresholds (<50 ng/mg).
  • Histological analysis confirmed ECM integrity and absence of nuclei in perfused scaffolds.

Conclusions:

  • A low-cost, reproducible gravity-assisted system generates high-quality acellular hepatic scaffolds without mechanical pumps.
  • This method is accessible and scalable for labs with limited infrastructure and educational purposes.
  • The approach supports future recellularization and preclinical studies for bioengineered liver constructs.