Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Structure-Activity Relationships and Drug Design01:28

Structure-Activity Relationships and Drug Design

1.6K
Drug design is a dynamic field that involves discovering and developing new medications based on specific biological targets. This process heavily relies on structure-activity relationships (SAR) and quantitative structure-activity relationships (QSAR) to guide the design and optimization of efficient drugs.
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence...
1.6K
RNA Editing02:23

RNA Editing

9.7K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.7K
Structural Protein Function01:56

Structural Protein Function

3.2K
3.2K
Structural Protein Function01:56

Structural Protein Function

29.7K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
29.7K
Conserved Binding Sites01:49

Conserved Binding Sites

5.0K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
5.0K
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

7.2K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PLASMOpred: A Machine Learning-Based Web Application for Predicting Antimalarial Small Molecules Targeting the Apical Membrane Antigen 1-Rhoptry Neck Protein 2 Invasion Complex.

Pharmaceuticals (Basel, Switzerland)·2025
Same author

Computer-Aided Discovery of Natural Compounds Targeting the ADAR2 dsRBD2-RNA Interface and Computational Modeling of Full-Length ADAR2 Protein Structure.

International journal of molecular sciences·2025
Same author

Natural Product Identification and Molecular Docking Studies of Leishmania Major Pteridine Reductase Inhibitors.

Pharmaceuticals (Basel, Switzerland)·2025
Same author

Design of Inhibitors That Target the Menin-Mixed-Lineage Leukemia Interaction.

Computation (Basel, Switzerland)·2024
Same author

ADAR Family Proteins: A Structural Review.

Current issues in molecular biology·2024
Same author

Identifying potential monkeypox virus inhibitors: an <i>in silico</i> study targeting the A42R protein.

Frontiers in cellular and infection microbiology·2024
Same journal

Correction: Wang et al. Phosphatidylserine Decarboxylase Promotes Ferroptosis Through STAT3/GPX4 Signaling in Gastric Cancer. <i>Curr. Issues Mol. Biol.</i> 2026, <i>48</i>, 300.

Current issues in molecular biology·2026
Same journal

Exploring the Relationship Between Protein-Level Ratios (rQLTs) and Duodenal Ulcer.

Current issues in molecular biology·2026
Same journal

Metformin as an Innate Immune Modulator: Metabolic and Epigenetic Reprogramming of Innate Immune Cells and Therapeutic Implications.

Current issues in molecular biology·2026
Same journal

Comprehensive Bioinformatic Characterization of CD70, CD80, and TIGIT as Diagnostic, Prognostic, and Immune Biomarkers in Pan-Cancer.

Current issues in molecular biology·2026
Same journal

Genome-Wide Identification and Expression Analysis of the Thaumatin-like Protein Genes in <i>Filipendula ulmaria</i> under <i>Bipolaris sorokiniana</i> Infection.

Current issues in molecular biology·2026
Same journal

Recent Dominant Transposition Events Affect Gene Regulatory Regions, but Not Coding Sequences, in Polar and Brown Bear Genomes.

Current issues in molecular biology·2026
See all related articles

Related Experiment Video

Updated: Jan 6, 2026

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
09:51

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

16.0K

In Silico Characterization of ADAR1: Structure, Dynamics, and Functional Implications.

Carolyn N Ashley1, Emmanuel Broni1, ChaNyah M Wood2

  • 1Department of Medicine, Loyola University Medical Center, Loyola University Chicago, Maywood, IL 60153, USA.

Current Issues in Molecular Biology
|November 26, 2025
PubMed
Summary
This summary is machine-generated.

Adenosine deaminase acting on RNA 1 (ADAR1) is vital for gene regulation. This study models full-length ADAR1, revealing its dynamic structure is key to RNA editing specificity and protein interactions, aiding therapeutic development.

Keywords:
ADAR1 enzymeRNA editingmolecular dynamics simulationsprincipal component analysis

More Related Videos

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.6K
Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function
05:57

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function

Published on: April 26, 2024

810

Related Experiment Videos

Last Updated: Jan 6, 2026

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
09:51

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

16.0K
Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.6K
Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function
05:57

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function

Published on: April 26, 2024

810

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Adenosine deaminase acting on RNA 1 (ADAR1) catalyzes A-to-I RNA editing, crucial for gene regulation in neural and immune systems.
  • ADAR1 dysregulation is linked to neurological disorders, cancer, and immune dysfunction, positioning it as a therapeutic target.
  • Lack of structural data for full-length ADAR1 and its dynamic behavior impedes therapeutic development.

Purpose of the Study:

  • To generate computational models of full-length ADAR1p150.
  • To analyze the dynamic behavior of ADAR1 and its impact on RNA editing specificity and protein interactions.
  • To provide a structural framework for understanding ADAR1 function and targeting it therapeutically.

Main Methods:

  • Homology modeling to create full-length ADAR1p150 models.
  • Molecular dynamics (MD) simulations to analyze protein dynamics.
  • Principal component analysis (PCA) and free-energy landscape mapping to identify conformational states.

Main Results:

  • ADAR1 models reveal stable dsRBD3 and CDD domains, essential for binding and catalysis.
  • ZBDs and dsRBD1/2 domains show significant flexibility, facilitating RNA recognition via conformational selection and fly-casting.
  • Free-energy landscapes highlight conserved domain cores and flexible loops, underscoring ADAR1's dynamic architecture.

Conclusions:

  • ADAR1's dynamic structure is critical for its RNA editing function and specificity.
  • The computational models and dynamic insights offer a framework for future ADAR1 research.
  • Understanding ADAR1 dynamics is essential for developing targeted therapies for associated diseases.