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Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

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Published on: October 29, 2015

HSBDF-Derived Bioactive Components Broadly Inhibit Enteroviruses by Targeting 3C Protease and Attenuating

Ruolan Hu¹, Lin Guan², Siyue Li²

¹School of Life Sciences, Tianjin University, Tianjin 300072, China.

|November 27, 2025

Summary

Huashi Baidu Formula (HSBDF), a traditional Chinese medicine, shows broad-spectrum antiviral activity against enteroviruses. Three flavonoids within HSBDF inhibit viral replication and inflammation, offering potential for new therapies.

Keywords:

3C protease inhibitor Huashi Baidu Formula (HSBDF)anti-inflammatory enterovirus flavonoids

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Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

Area of Science:

Virology
Pharmacology
Traditional Chinese Medicine

Background:

Human enteroviruses cause significant global health issues like hand-foot-and-mouth disease and encephalitis, with no specific treatments.
Traditional Chinese Medicine (TCM) showed antiviral promise during COVID-19, but its anti-enterovirus effects are not well understood.

Purpose of the Study:

To investigate the anti-enterovirus activity of Huashi Baidu Formula (HSBDF), a TCM used for COVID-19.
To identify active compounds in HSBDF and elucidate their anti-enterovirus mechanisms.

Main Methods:

Systematic characterization of HSBDF's anti-enterovirus activity.
Transcriptomics and High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS) analyses.
In vitro assays on viral protease activity and inflammatory markers.
In vivo studies using a mouse model.

Main Results:

HSBDF demonstrated broad-spectrum anti-enterovirus activity.
Three flavonoids (velutin, isorhamnetin, (-)-epicatechin gallate) were identified as key active components.
HSBDF modulated MAPK and NF-κB pathways, reducing CV-A9-induced inflammation.
These flavonoids inhibited enterovirus 3C protease activity and key inflammatory mediators.
HSBDF and velutin suppressed viral replication in a mouse model.

Conclusions:

HSBDF is a promising TCM-based strategy for enterovirus therapy.
Flavonoid compounds show potential as broad-spectrum anti-enteroviral agents.