Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.5K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.5K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

8.1K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
8.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early photoperiodic cues modulate capacity for adult cold tolerance in the yellow fever mosquito Aedes aegypti.

The Journal of experimental biology·2026
Same author

Leveraging learned representations and multitask learning for lysine methylation site discovery.

Scientific reports·2026
Same author

Multimeric Branched and Hyperbranched Peptide Ligands of the Natural Cytotoxicity Receptor NKp30 on Natural Killer Cells.

Bioconjugate chemistry·2026
Same author

Machine learning-driven prediction of substrates for enzymes introducing or removing protein post-translational modifications.

Communications chemistry·2025
Same author

Exploration into the MLL4/WRAD Enzyme-Substrate Network: Systematic In Vitro Identification of CFP1 as a Potential Non-Histone Substrate of the MLL4 Lysine Methyltransferase.

Epigenomes·2025
Same author

Sequence-Based Protein-Protein Interaction Prediction and Its Applications in Drug Discovery.

Cells·2025

Related Experiment Video

Updated: Jan 10, 2026

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

2.9K

Mass Spectrometry for Lysine Methylation: Principles, Progress, and Prospects.

Mackenzie G Cumming1, Kyle K Biggar1

  • 1Institute of Biochemistry, Carleton University, Ottawa, ON K2J0L6, Canada.

Biomedicines
|November 27, 2025
PubMed
Summary
This summary is machine-generated.

Lysine methylation analysis using mass spectrometry (MS) is challenging but advancing. This review details MS workflow evolution for methyllysine proteomics, addressing sensitivity and specificity improvements.

Keywords:
lysine methylationmass spectrometrymethyllysineproteomics

More Related Videos

Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity
07:02

Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity

Published on: March 11, 2012

13.7K
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

14.4K

Related Experiment Videos

Last Updated: Jan 10, 2026

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

2.9K
Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity
07:02

Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity

Published on: March 11, 2012

13.7K
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

14.4K

Area of Science:

  • Biochemistry
  • Proteomics
  • Molecular Biology

Background:

  • Lysine methylation is a crucial regulatory post-translational modification in proteins.
  • Its low stoichiometry and subtle mass shifts present significant analytical challenges.
  • Current enrichment strategies for methyllysine are not standardized or robust.

Purpose of the Study:

  • To review the advancements in mass spectrometry (MS)-based workflows for lysine methylation analysis.
  • To evaluate different proteomic approaches and enrichment techniques.
  • To identify persistent challenges and future directions in methyllysine profiling.

Main Methods:

  • Review of mass spectrometry techniques including ionization, fragmentation, and acquisition strategies (DIA, PASEF).
  • Evaluation of bottom-up, middle-down, and top-down proteomic approaches.
  • Discussion of enrichment methods: immunoaffinity, chromatography, and chemical derivatization.

Main Results:

  • Significant evolution in MS technology has improved lysine methylation analysis.
  • Various proteomic strategies and enrichment methods offer different advantages and limitations.
  • Proteolytic constraints and isobaric interference remain key challenges for site-level resolution.

Conclusions:

  • Optimizing MS workflows is critical for sensitive, specific, and reproducible methyllysine profiling.
  • Emerging solutions and future research directions are needed to overcome current limitations.
  • This review provides a roadmap for advancing methyllysine proteomics.