Different HLA Alleles Frequencies and Their Association with Clinical Phenotypes of Acute Respiratory Infections in...

The histocompatibility gene complex plays a vital role in the body's immune response to infections. In this work, we analyzed clinical data for 195 children hospitalized with signs of ARI in Siberia and performed genetic analysis for them. Genotyping was performed by high-throughput sequencing (NGS) using the HLA-Expert kit on the MiSeq Illumina platform. The frequencies of HLA allelic variants were calculated for each variant. For the variants detected in 20 patients or more, odds ratios (OR) were calculated for two pairs of conditions: severe/non-severe course of ARI and hypoxia/no hypoxia on admission. Six allelic variants were identified for which the odds ratio showed a significant (p < 0.05) association with one of the conditions. Allele HLA-A*11:01:01G is associated (OR = 5.654, 95% CI 1.631-19.600) with severe ARVI, which is consistent with the literature data, and HLA-A*03:01:01G allele is associated with ARVI without hypoxia in children (OR = 0.317, 95% CI 0.110-0.914). Alleles HLA-B*51:01:01G (OR = 4.457, 95% CI 1.355-14.663) and HLA-C*01:02:01G (OR = 4.743, 95% CI 1.538-14.629) are associated with severe ARI. HLA-DPB1*04:02:01G (OR = 0.462, 95% CI 0.244-0.876) is associated with ARI without hypoxia and HLA-DQA1*01:02:01G (OR = 1.811, 95% CI 1.003-3.268) is associated with ARI with hypoxia.