Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Placental Site Trophoblastic Tumor Acquires Immune Functions by Incorporating Host Maternal Genes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

HLA-DRB3/4/5-based susceptibility profiles in nivolumab-induced type 1 diabetes and interstitial lung disease.

Frontiers in immunology·2026

Same author

Systems-level investigation of the anxiolytic gut-brain interactions induced by paraprobiotic <i>Lactobacillus brevis</i> SBC8803 in zebrafish.

Frontiers in microbiology·2026

Same author

IL-6 and TNFα are associated with depressive symptoms among men in a community-based cohort, with a tentative trend for IL-17A: Findings from the Shika Study in Japan.

Neuro endocrinology letters·2026

Same author

Epidemiological impact of a 20- or 21-valent pneumococcal conjugate vaccine in the Japan adult population: insights from a dynamic transmission model.

Expert review of vaccines·2026

Same author

Polygenic risk scores and lifestyle factors predicting new onset of type 2 diabetes in the Japanese general population.

The Journal of clinical endocrinology and metabolism·2026

Search research articles

Related Experiment Video

Updated: Jan 10, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

Click-qPCR: A Simple Tool for Interactive qPCR Data Analysis.

Azusa Kubota¹, Atsushi Tajima¹

¹Department of Bioinformatics and Genomics, Graduate School of Advanced Preventive Medical Sciences, Kanazawa University, Kanazawa, Japan.

|November 27, 2025

Summary

This summary is machine-generated.

Click-qPCR is a free, web-based tool that simplifies gene expression analysis using real-time quantitative PCR (qPCR) data. This application enables biologists to easily perform complex statistical analyses and generate publication-quality visualizations without coding skills.

Keywords:

Bioinformatics Copy number variation analysis Gene expression analysis Real-time quantitative PCR (qPCR)Shiny web application

More Related Videos

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

Published on: March 6, 2019

Related Experiment Videos

Last Updated: Jan 10, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

Published on: March 6, 2019

Area of Science:

Molecular Biology
Bioinformatics
Genomics

Background:

Real-time quantitative PCR (qPCR) is essential for gene expression and DNA copy number analysis.
Limited user-friendly software hinders qPCR data analysis for biologists lacking computational expertise.

Purpose of the Study:

To develop Click-qPCR, an accessible, web-based Shiny application for streamlined qPCR data analysis.
To empower researchers with efficient tools for processing, interpreting, and visualizing qPCR results.

Main Methods:

Developed a user-friendly, browser-based Shiny application named Click-qPCR.
Integrated ΔCq and ΔΔCq calculations from user-uploaded CSV files.
Implemented interactive selection of genes/groups for Welch's t-tests and ANOVA with Dunnett's post-hoc test.

Main Results:

Click-qPCR provides intuitive workflows for statistical analysis of qPCR data.
Interactive bar plots with mean ± SD and individual data points are generated.
Publication-quality visualizations and summary statistics are downloadable.

Conclusions:

Click-qPCR facilitates efficient and reproducible qPCR data analysis for researchers of all skill levels.
The open-source, freely accessible application promotes transparency and adoption in molecular biology and biomedical research.