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Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

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Published on: June 1, 2012

DNA-based Bi-layered effervescent ejecting microneedle (BEE MN) for glucose-responsive insulin delivery.

Yoonbin Ji¹, Kyung A Kim¹, Iksoo Jang¹

¹Department of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemun-gu, Seoul 02504, Republic of Korea. jblee@uos.ac.kr.

Materials Horizons

|November 27, 2025

Summary

This study introduces a novel bi-layered effervescent ejecting microneedle (BEE MN) system that uses CO2 gas for self-propulsion. This innovation enhances transdermal drug delivery, offering efficient, on-demand insulin administration for diabetes management.

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Area of Science:

Biomaterials Science
Drug Delivery Systems
Nanotechnology

Background:

Conventional injections face limitations in drug loading and release for chronic diseases.
Microneedle technology offers minimally invasive alternatives but requires enhanced delivery mechanisms.

Purpose of the Study:

To develop a novel bi-layered effervescent ejecting microneedle (BEE MN) system for enhanced transdermal drug delivery.
To engineer a glucose-responsive system for precise, on-demand insulin administration.

Main Methods:

Designed a dual-stage microneedle with a glucose-responsive DNA reservoir and an effervescent matrix for CO2 generation.
Utilized rolling circle amplification (RCA) to create insulin-binding aptamer structures.
Evaluated drug penetration, retention, and glucose-triggered release in vitro.

Main Results:

The BEE MN system demonstrated CO2-powered self-propulsion, improving drug delivery into the dermal layer.
Engineered DNA reservoirs enabled high-density aptamer structures for controlled insulin release.
Achieved efficient, glucose-responsive insulin release under hyperglycemic conditions.

Conclusions:

The CO2-powered, self-propulsive microneedle system offers enhanced transdermal drug delivery capabilities.
This technology presents a promising platform for next-generation smart insulin delivery systems.
The BEE MN system overcomes limitations of traditional microneedles for efficient, on-demand drug administration.