Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Programmable Double Peptide Nucleic Acid-Locked Nucleic Acid Molecular Switch Enables Extraction-Free Direct Zygosity Discrimination of Single Nucleotide Polymorphisms.

Analytical chemistry·2026

Same author

Integrated Biodevice for Parallel Magnetic Isolation and Bioelectrocatalytic Detection of Circulating DNA and RNA Biomarkers in Non-Small Cell Lung Cancer Liquid Biopsy.

ACS sensors·2026

Same author

Alarming disparities of adolescent motherhood in Nigeria: results from 36 states and the Federal Capital Territory.

BMJ global health·2026

Same author

The need to strengthen Australian health and social policy to support survivors of the Stolen Generations.

BMJ global health·2026

Same author

Repulsive Gas-Electrode van der Waals Forces Enable Charge Transfer Reactions under Chemically Modified Bubbles.

Journal of the American Chemical Society·2026

Same author

Biomolecule Detection Methods Based on Nanoparticle Approaches.

ACS omega·2026

Search research articles

Related Experiment Video

Updated: Apr 6, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Challenges in the Development and Implementation of Point-of-Care Handheld qPCR Devices.

Kiran Shrestha¹, Fatema Zerin Farhana¹, Kevin M Koo²

¹Rural Health Research Institute, Charles Sturt University, Orange, NSW, 2800, Australia.

Small (Weinheim an Der Bergstrasse, Germany)

|November 27, 2025

Summary

This summary is machine-generated.

Developing point-of-care quantitative PCR (qPCR) requires overcoming challenges in sample preparation and device miniaturization. Innovations in microfluidics and nanoparticles are key to creating rapid, cost-effective, and user-friendly nucleic acid detection platforms for field use.

Keywords:

handheld diagnostic devices microfluidics miniaturization molecular diagnostics nucleic acid detection point‐of‐care (POC) devices portable PCR quantitative PCR (qPCR)

More Related Videos

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Published on: June 17, 2022

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Related Experiment Videos

Last Updated: Apr 6, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Published on: June 17, 2022

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Area of Science:

Biotechnology
Molecular Diagnostics
Point-of-Care Testing

Background:

Polymerase chain reaction (PCR) is crucial for nucleic acid amplification and detection.
Its applications span diagnostics, environmental monitoring, agriculture, and forensics.
Translating laboratory PCR to point-of-care quantitative PCR (qPCR) is essential but challenging.

Purpose of the Study:

To review challenges and approaches for developing point-of-care quantitative PCR (qPCR) platforms.
To highlight innovations in sample preparation and device miniaturization.
To discuss strategies for cost reduction and standardization for widespread adoption.

Main Methods:

Review of recent advancements in sample preparation using microfluidics and nanoparticles.
Examination of thermal cycler and nucleic acid detection method development for miniaturization.
Analysis of strategies for cost reduction and standardization.

Main Results:

Microfluidics and nanoparticles offer speed, efficiency, and simplicity in nucleic acid extraction.
Device miniaturization faces hurdles in thermal cycling and detection.
Cost reduction and standardization are critical for POC qPCR adoption.

Conclusions:

Simplified sample preparation and miniaturized qPCR devices are vital for on-site implementation.
Addressing challenges in thermal cyclers and detection is necessary for effective field devices.
Developing rapid, precise, handheld, and low-cost POC qPCR devices requires practical considerations and innovation.