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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Updated: Jan 10, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Nanopore-Based Label-Free and Single-Molecule Sequencing toward Precision Diagnosis.

Yufan Cui1,2, Yueming Zhai3, Yongxi Zhao4,5

  • 1School of Biomedical Engineering, School of Science, School of Marine Science and Technology & Key Laboratory of Science and Engineering for the Multi-modal Prevention and Control of Major Chronic Diseases, Ministry of Industry and Information Technology, Harbin Institute of Technology, Shenzhen, Guangdong, 518055, China.

Advanced Materials (Deerfield Beach, Fla.)
|November 27, 2025
PubMed
Summary

Nanopore sequencing (SMS) offers detailed single-molecule analysis of biomolecules for precision diagnostics. This review covers nanopore technology, DNA/protein sequencing, and clinical applications, highlighting future potential.

Keywords:
DNA sequencingnanoporesprecision diagnosisprotein sequencingsingle‐molecule sensing

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Nanotechnology

Background:

  • Nanopore-based single-molecule sequencing (SMS) provides high-resolution analysis of biomolecules.
  • Real-time current fluctuations enable long-read, high-throughput, and accurate detection for precision diagnosis.

Purpose of the Study:

  • To provide an overview of nanopore principles and fabrication methods.
  • To highlight advancements in nanopore DNA and protein sequencing.
  • To analyze clinical applications and future perspectives of nanopore SMS.

Main Methods:

  • Review of nanopore sequencing principles and fabrication techniques.
  • Analysis of innovative molecule capture and translocation strategies.
  • Discussion of strategies for enhancing nanopore performance.

Main Results:

  • Recent advancements in nanopore-based DNA and protein sequencing.
  • Successful applications in diagnosing genetic disorders, infectious diseases, and cancers.
  • Identification of limitations and strategies for performance enhancement.

Conclusions:

  • Nanopore SMS holds significant clinical potential for precision diagnosis.
  • Future developments will integrate microfluidics, surface functionalization, and machine learning.
  • Continued research is essential for advancing nanopore technology.