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Updated: Jan 10, 2026

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
Published on: July 3, 2013
Propofol Suppresses Early Afterdepolarization in Isoproterenol-Stimulated Cardiomyocytes via L-Type Calcium Current
1Department of Cardiology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Objective:
This study aims to investigate the effects of short-term propofol application on the electrophysiological properties of ventricular myocytes under the stress conditions.
Methods:
A stress model was established in adult guinea pig ventricular myocytes using isoproterenol. Whole-cell patch clamp techniques were used to systematically assess the dose-dependent effects of propofol at concentrations of 30 μM, 100 μM, and 200 μM on action potential characteristics, triggered activity, and key ionic currents (INa-L, ICa-L, and IKr).
Results:
Propofol dose-dependently suppressed the ICa-L, significantly shortened the plateau phase of the action potential, and reduced the frequency of early afterdepolarization. Propofol showed no significant effect on INa-L or IKr.
Conclusion:
These findings indicate that propofol may exert potential anti-arrhythmic effects against stress-induced ventricular arrhythmias by inhibiting triggered activity.
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