Related Experiment Video

Updated: Jan 10, 2026

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

1.1K

TGM2 Regulates Radiosensitivity via POGZ-Mediated Repair of DNA Double-Strand Breaks in Cervical Cancer

Yunbo Chi1, Peng Dong2, Ning Zhang1

  • 1Department of Radiation Oncology, China-Japan Union Hospital of Jilin University, Changchun, China.

Cancer Science
|November 27, 2025
PubMed

Insights

Transglutaminase 2 (TGM2) promotes radioresistance in cervical cancer by enhancing DNA repair. Targeting TGM2 and its interaction with POGO transposable element derived with ZNF domain protein (POGZ) may improve cancer treatment outcomes.

Area of Science:

  • Oncology
  • Molecular Biology
  • Radiotherapy Research

Background:

  • Cancer cell radioresistance is a major challenge in treatment, often linked to DNA repair mechanisms.
  • Aberrant expression of transglutaminase 2 (TGM2) is associated with treatment resistance, but its role in cervical cancer radiosensitivity is unknown.

Purpose of the Study:

  • To investigate the role of TGM2 in cervical cancer radiosensitivity and elucidate its underlying molecular mechanisms.

Main Methods:

  • Quantitative analysis of TGM2 expression in radioresistant cervical cancer cells and tissues.
  • In vitro studies involving TGM2 knockdown and overexpression to assess radiosensitivity.
  • Investigation of TGM2's interaction with POGO transposable element derived with ZNF domain protein (POGZ) and BRCA1 recruitment to DNA double-strand breaks (DSBs) after ionizing radiation (IR).
  • In vivo validation using subcutaneous xenograft mouse models.

Main Results:

  • TGM2 was significantly upregulated in radioresistant cervical cancer cells and tissues.
  • TGM2 knockdown enhanced radiosensitivity, while overexpression conferred resistance.
  • TGM2 depletion increased IR-induced DNA DSBs and impaired BRCA1 recruitment, similar to POGZ depletion.
  • POGZ knockdown reversed the effects of TGM2 overexpression on radioresistance and DSB repair.
  • In vivo studies confirmed TGM2's role in regulating cervical cancer radiosensitivity.

Conclusions:

  • TGM2 promotes cervical cancer radioresistance by enhancing POGZ-mediated DNA DSB repair.
  • TGM2 represents a potential therapeutic target for overcoming radioresistance in cervical cancer.
Keywords:
DNA double‐strand breaksPOGZTGM2cervical cancerradiosensitivity

More Related Videos

Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry08:21

Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry

Published on: September 1, 2019

14.1K
Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.1K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.9K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.0K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.2K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.3K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K