Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A missing link for efflux pumps.

eLife·2026
Same author

Genetic and genomic variability of <i>Spiroplasma</i> and <i>Midichloria</i> endosymbionts associated with the tick <i>Ixodes frontalis</i>.

ISME communications·2025
Same author

MexB/inhibitor complexes preparation for crystallography and cryo-EM, comparison and possible pitfalls.

Methods in enzymology·2025
Same author

Broadening the semiaquatic scene: Quantification of long bone microanatomy across pinnipeds.

Anatomical record (Hoboken, N.J. : 2007)·2025
Same author

An alternative mechanism by which If1 prevents ATP hydrolysis by the ATP synthase subcomplex in S. cerevisiae.

EMBO reports·2025
Same author

Surviving a Dark Age: The Oldest Baleen-Bearing Whales (Cetacea: Chaeomysticeti) of Pacific South America (Lower Miocene, Peru).

Life (Basel, Switzerland)·2025

Related Experiment Video

Updated: Jan 10, 2026

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
12:10

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Published on: March 28, 2011

23.9K

Reconstitution of RND tripartite multidrug complexes for single-particle electron microscopy.

Esther Boyer1, Laetitia Daury1, Marie-France Giraud1

  • 1Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR5248, Pessac, France.

Methods in Enzymology
|November 27, 2025
PubMed
Summary

Tripartite multidrug efflux pumps (TEPs) in Gram-negative bacteria assemble using inner membrane transporters, outer membrane factors, and periplasmic adaptor proteins. This study reveals molecular recognition rules governing TEP component assembly and function.

Keywords:
Electron microscopyMembrane proteinMultidrug efflux pumpNanodiscSingle particle analysis

More Related Videos

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

9.9K
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
13:28

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Published on: May 16, 2017

50.8K

Related Experiment Videos

Last Updated: Jan 10, 2026

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
12:10

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Published on: March 28, 2011

23.9K
Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

9.9K
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
13:28

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Published on: May 16, 2017

50.8K

Area of Science:

  • Microbiology
  • Structural Biology
  • Biochemistry

Background:

  • Gram-negative bacteria utilize tripartite efflux pumps (TEPs) for multidrug resistance.
  • TEPs comprise an RND transporter, an outer membrane factor (OMF), and a periplasmic adaptor protein (PAP).
  • The assembly mechanism and partner selection rules for TEPs remain poorly understood.

Purpose of the Study:

  • To investigate the molecular recognition and assembly of TEP components.
  • To understand the rules governing partner selection in TEP formation.
  • To elucidate the impact of factors like pH on TEP assembly.

Main Methods:

  • Reconstitution of Pseudomonas aeruginosa OprM-MexA-MexB TEP and chimeric OprN-MexA-MexB TEP in nanodiscs.
  • Structural characterization using single-particle electron microscopy.
  • Assessment of TEP formation with cognate and non-cognate partners.

Main Results:

  • Successful reconstitution of TEPs in nanodiscs allowed controlled assessment of component interactions.
  • Insights into the molecular recognition governing TEP assembly were obtained.
  • The influence of pH and other factors on TEP assembly was elucidated.

Conclusions:

  • The study provides a method to assess TEP formation and component interactions.
  • Understanding TEP assembly mechanisms can inform strategies to combat multidrug resistance in bacteria.
  • This work sheds light on the flexibility and specificity of TEP component interactions.