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The diagnostic and prognostic value of circulating miR-193b-3p on severe pneumonia
Kunbin Liu1, Ping Wang2, Yuan Li3
1Department of Intensive Care Unit, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin, 300060, China.
Background:
Severe pneumonia (SP) poses a serious threat to patients' lives, and this study aimed to explore the diagnostic and prognostic value of miR-193b-3p on SP, thereby providing novel insights for the clinical management of SP.
Methods:
The miR-193b-3p expression was evaluated by PCR. The diagnostic value of miR-193b-3p on SP was assessed by ROC. The association between miR-193b-3p expression and the SP severity was assessed by the correlation analysis. The correlation between miR-193b-3p expression and the survival status of SP patients was evaluated by the Kaplan-Meier survival analysis. Potential independent prognostic factors for SP were predicted by multivariate COX regression analysis. Bioinformatics analysis assessed the possible pathways regulated by miR-193b-3p.
Results:
Upregulation of miR-193b-3p in SP patients showed a diagnostic value in SP. The miR-193b-3p expression was positively correlated with the levels of WBC, NEU, CRP, PCT, BNP, and D-D, and negatively correlated with LYM. The expression level of miR-193b-3p was significantly associated with the APACHE-II and CPIS scores, and a better survival rate was observed in SP subjects with low miR-193b-3p expression levels. MiR-193b-3p, together with CRP, PCT, APACHE-II, and CPIS, could also serve as independent prognostic factors for SP. MiR-193b-3p was associated with PI3K-Akt, MAPK, FoxO, TRP channel, and Wnt signaling pathways.
Conclusion:
Upregulated miR-193b-3p expression in SP patients had a diagnostic and prognostic value for SP.
Insights
Upregulated miR-193b-3p microRNA shows diagnostic and prognostic value in severe pneumonia (SP). Lower miR-193b-3p expression correlates with better survival rates in SP patients, aiding clinical management.
Area of Science:
- Biochemistry
- Molecular Biology
- Medical Diagnostics
Background:
- Severe pneumonia (SP) is a life-threatening condition.
- Effective diagnostic and prognostic biomarkers are crucial for managing SP.
- miR-193b-3p is investigated for its potential role in SP.
Purpose of the Study:
- To evaluate the diagnostic value of miR-193b-3p in SP.
- To assess the prognostic significance of miR-193b-3p in SP patients.
- To explore the correlation between miR-193b-3p expression and SP severity and survival.
Main Methods:
- Quantitative PCR (qPCR) for miR-193b-3p expression.
- Receiver Operating Characteristic (ROC) curve analysis for diagnostic accuracy.
- Correlation analysis, Kaplan-Meier survival analysis, and multivariate COX regression for prognostic evaluation.
- Bioinformatics analysis to identify regulated pathways.
Main Results:
- Upregulated miR-193b-3p expression in SP patients demonstrated diagnostic value.
- miR-193b-3p levels correlated positively with WBC, NEU, CRP, PCT, BNP, D-D and negatively with LYM.
- Lower miR-193b-3p expression was associated with better survival and lower severity scores (APACHE-II, CPIS).
- miR-193b-3p, CRP, PCT, APACHE-II, and CPIS identified as independent prognostic factors.
- miR-193b-3p is implicated in PI3K-Akt, MAPK, FoxO, TRP channel, and Wnt signaling pathways.
Conclusions:
- Upregulated miR-193b-3p expression serves as a valuable diagnostic marker for SP.
- miR-193b-3p expression is a significant prognostic indicator for SP outcomes.
- These findings offer insights for improved clinical management of severe pneumonia.
