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Protocol for quantifying coccidian parasite mechanics and rupture force using advanced micromanipulation-based

Jana El Husseiny1, Pierre-Henri Puech1, Aurélien Dumètre1

  • 1Adhesion and Inflammation Laboratory (LAI), Aix Marseille Université, CNRS, Inserm, 13288 Marseille, France.

STAR Protocols
|November 28, 2025
PubMed
Summary

This study introduces a new microindentation method to measure the mechanical strength of coccidian parasite oocyst walls. This technique quantifies rupture forces, aiding in understanding parasite infection mechanics.

Keywords:
BiophysicsMicrobiologymicroscopy

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Area of Science:

  • Parasitology
  • Biophysics
  • Materials Science

Background:

  • Coccidian parasites utilize mechanical properties for host infection.
  • Understanding oocyst wall mechanics is crucial for parasite control.

Purpose of the Study:

  • To develop and validate a microindentation technique for quantifying coccidian oocyst wall mechanics.
  • To enable precise measurement of oocyst wall rupture forces.

Main Methods:

  • Fabrication and calibration of flexible microindenters using micropipettes.
  • Assembly of a microindentation system for force measurement.
  • Automated data analysis using open-source software and programming.

Main Results:

  • The developed protocol accurately quantifies coccidian oocyst wall indentation and rupture forces.
  • The technique is robust and reproducible for mechanical property assessment.

Conclusions:

  • This microindentation method provides a quantitative approach to study parasite oocyst wall integrity.
  • The protocol is adaptable for investigating parasite mechanics under diverse physiological conditions.