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Published on: October 23, 2018
MiR-18a-5p serves as a risk factor in perioperative respiratory adverse events in children under general anesthesia
Lin Zhang1, Xinyuan Yu2, Jun Liu3
1The First Clinical Medical College, Heilongjiang University of Chinese Medicine, Harbin, 150400, China.
Insights
This study found that miR-18a-5p is a significant predictor and diagnostic biomarker for perioperative respiratory adverse events (PRAEs) in pediatric anesthesia. Its levels correlate with inflammation and patient factors, suggesting therapeutic potential.
Area of Science:
- Pediatric Anesthesiology
- Molecular Biology
- Biomarker Discovery
Background:
- Perioperative respiratory adverse events (PRAEs) are common in pediatric anesthesia.
- Identifying reliable biomarkers for PRAEs is crucial for clinical management.
Purpose of the Study:
- To investigate the role of miR-18a-5p as a potential biomarker for PRAEs in children.
- To explore the diagnostic and predictive value of miR-18a-5p in pediatric anesthesia.
Main Methods:
- Analysis of clinical data from 174 children undergoing general anesthesia.
- Quantification of miR-18a-5p expression using qRT-PCR in serum and cells.
- Evaluation of diagnostic value via ROC curves and identification of risk factors using logistic regression.
- In vitro experiments assessing miR-18a-5p effects on lung cells and inflammatory markers.
Main Results:
- Elevated miR-18a-5p levels were observed in children with PRAEs, showing diagnostic relevance.
- miR-18a-5p expression positively correlated with TNF-α and IL-6 levels.
- Age, obesity, ASA grade, and anesthesia duration were identified as independent risk factors for PRAEs.
- miR-18a-5p silencing improved lung cell viability and reduced inflammatory responses.
Conclusions:
- miR-18a-5p is a significant predictive and diagnostic factor for PRAEs in pediatric general anesthesia.
- The findings suggest miR-18a-5p may hold therapeutic potential for managing PRAEs.
Purpose:
Perioperative respiratory adverse events (PRAEs) are frequently encountered complications in pediatric anesthesia. The purpose of this experiment is to explore the significant role of miR-18a-5p in PRAEs, thereby providing a novel biomarker for PRAE clinical management.
Methods:
Clinical data from 174 children undergoing general anesthesia were analyzed. MiR-18a-5p expression in serum and cells was analyzed via real-time quantitative PCR (qRT-PCR). Its diagnostic value for PRAEs was evaluated through receiver operating characteristic (ROC) curves, while logistic regression was selected to assess independent risk factors for PRAEs. In vitro experiments were conducted to determine miR-18a-5p effects on AEC-II cells proliferation, apoptosis, and lipopolysaccharide (LPS)-induced tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) secretion.
Results:
PRAEs subjects exhibited significantly elevated miR-18a-5p levels with diagnostic relevance. Expression correlated positively with TNF-α/IL-6 concentrations and was associated with age, obesity, American Society of Anesthesiologists (ASA) grade, and anesthesia duration. These parameters jointly constituted the PRAEs predictors. Furthermore, miR-18a-5p silencing enhanced AEC-II cells' viability while suppressing apoptosis and LPS-triggered TNF-α and IL-6 release.
Conclusion:
The miR-18a-5p served as a predictive factor and held diagnostic significance for PRAEs following general anesthesia in children. Additionally, miR-18a-5p may offer potential therapeutic implications for PRAEs.
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