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Updated: Jan 10, 2026
Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
Published on: January 26, 2017
Protocol for systematic screening of histone H3/H4 mutants to identify histone residues critical for BCAA-dependent
Sejung Park1, Yan Liu1, Seong Hoon Ahn1
1Major in Molecular Medicine, School of Biopharmaceutical Convergence, College of Advanced Technology and Convergence, Hanyang University, Ansan, Gyeonggi-do 15588, Republic of Korea.
Abstract:
Here, we present a protocol for high-throughput replicative lifespan (RLS) screening in Saccharomyces cerevisiae using micromanipulation-based aging assays with growth phenotyping. We describe steps for preparing yeast strains, isolating virgin cells, and monitoring divisions to calculate lifespan changes in histone H3/H4 mutants. We also describe sulfometuron methyl (SM) spotting assay to evaluate growth phenotypes linked to branched-chain amino acid (BCAA) metabolism, enabling identification of residues critical for lifespan regulation. For complete details on the use and execution of this protocol, please refer to Park et al.1.
