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CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Updated: Jan 9, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

CRISPR screen-based mammalian cell engineering for complex biotherapeutics.

Sung Wook Shin¹, Gyun Min Lee², Jae Seong Lee³

¹Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Republic of Korea.

Trends in Biotechnology

|November 29, 2025

Summary

This summary is machine-generated.

Clustered regularly interspaced short palindromic repeats (CRISPR) screens help identify targets to improve biomanufacturing. This approach addresses bottlenecks in producing complex biotherapeutics using mammalian cells.

Keywords:

CRISPR screen biomanufacturing difficult-to-express functional genomics mammalian cell engineering novel biotherapeutics

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Last Updated: Jan 9, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

Area of Science:

Biotechnology
Molecular Biology
Bioprocessing

Background:

Complex biotherapeutics production relies heavily on mammalian cell lines.
Current biomanufacturing processes face significant bottlenecks, hindering efficient production.
There is a need for advanced methods to optimize cell factories for biopharmaceutical manufacturing.

Purpose of the Study:

To provide an overview of CRISPR-based screening applications in biomanufacturing.
To highlight recent advancements in using CRISPR technology for cell engineering.
To discuss challenges and future directions in developing robust cell factories.

Main Methods:

Utilizing Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based screening.
Employing unbiased discovery approaches for engineering targets.
Focusing on mitigating biomanufacturing-relevant constraints in mammalian cells.

Main Results:

CRISPR screens enable the identification of key targets for biomanufacturing optimization.
These screens facilitate the discovery of strategies to overcome production limitations.
Advances in CRISPR technology are paving the way for improved cell factory design.

Conclusions:

CRISPR screening is a powerful tool for advancing biotherapeutic production.
Addressing remaining challenges will further enhance the development of specialized cell factories.
The application of CRISPR technology promises more robust and efficient biomanufacturing processes.