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Updated: Jan 9, 2026
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Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Published on: September 4, 2019
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CRISPR screen-based mammalian cell engineering for complex biotherapeutics
Sung Wook Shin1, Gyun Min Lee2, Jae Seong Lee3
1Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Republic of Korea.
Trends in Biotechnology
|November 29, 2025
Abstract
None:
The rise of complex biotherapeutics has introduced bottlenecks in production using mammalian cells. Clustered regularly interspaced short palindromic repeats (CRISPR)-based screens enable unbiased discovery of engineering targets that mitigate biomanufacturing-relevant constraints. This forum gives an overview of recent advances and remaining challenges in applying CRISPR screening to build robust, modality-specific cell factories.
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