Related Experiment Video

Updated: Jan 9, 2026

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
06:18

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

Published on: March 3, 2023

1.7K

Different media affect the growth and antimicrobial minimum inhibitory concentrations of Mycoplasma gallisepticum and

Hossein Taiyari1, Jalila Abu1, Zunita Zakaria2

  • 1Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Journal of Microbiological Methods
|November 30, 2025
PubMed

Insights

The growth medium significantly impacts Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) growth rates and antibiotic susceptibility testing. Frey medium promoted faster growth and altered minimum inhibitory concentration (MIC) values compared to PPLO medium for these avian pathogens.

Area of Science:

  • Avian Health
  • Microbiology
  • Veterinary Pharmacology

Background:

  • Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are key pathogens causing chronic respiratory disease in chickens.
  • Antibiotic therapy is a primary control method for avian mycoplasmosis.
  • The minimum inhibitory concentration (MIC) assay determines antibiotic susceptibility, but the influence of growth media on MG and MS MIC values is unexamined.

Purpose of the Study:

  • To investigate the impact of Frey medium versus Pleuropneumonia-like Organisms (PPLO) medium on the growth rate of MG and MS.
  • To determine how these two media affect the MIC values of MG and MS isolates.
  • To evaluate the influence of growth media on antibiotic susceptibility testing for avian mycoplasmosis.

Main Methods:

  • Comparative analysis of MG and MS growth rates in Frey and PPLO media across three replicates.
  • Determination of antimicrobial MIC values for MG and MS isolates using the microbroth dilution method.
  • Statistical comparison of growth rates and MIC values between the two tested media.

Main Results:

  • Both MG and MS isolates exhibited significantly faster growth rates in Frey medium compared to PPLO medium (p ≤ 0.05).
  • Antimicrobial MIC values for MG and MS isolates varied depending on whether Frey or PPLO medium was used.
  • The type of growth medium has a significant effect on the determined MIC values for these important avian pathogens.

Conclusions:

  • The choice of growth medium (Frey vs. PPLO) critically influences the growth characteristics and antibiotic susceptibility profiles of Mycoplasma gallisepticum and Mycoplasma synoviae.
  • Standardized protocols for avian mycoplasmosis MIC testing should consider the impact of growth media.
  • This study provides foundational data for developing more accurate and comprehensive MG and MS MIC testing protocols.
Keywords:
Avian mycoplasmosisFrey mediumGrowth rateM. gallisepticumM. synoviaeMinimum inhibitory concentrationPPLO medium

More Related Videos

Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition07:42

Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition

Published on: September 3, 2016

15.4K
Growth of Mycobacterium tuberculosis Biofilms09:03

Growth of Mycobacterium tuberculosis Biofilms

Published on: February 15, 2012

24.4K

Related Concept Videos

Microbial Growth Media01:27

Microbial Growth Media

Microbial growth media are essential tools in microbiology, providing the nutrients and conditions necessary to cultivate and study microorganisms. These media are categorized by their composition, consistency, and functional roles, enabling researchers to investigate microbial physiology, behavior, and interactions.Types and Consistencies of Growth MediaGrowth media can be solid, liquid, or semisolid. Solid media, often agar-based, allow visible colony growth for isolation and enumeration.
1.2K
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
883
Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
1.3K