Analytical Methods for Daptomycin Determination: Applications in Biological Matrices and Pharmaceutical Formulations
Zeynep Derya Bilgin1, Sıdıka Ertürk Toker2
1Analytical Chemistry Doctorate Program, Institute of Graduate Studies in Health Sciences, Istanbul University, Istanbul, Türkiye.
Critical Reviews in Analytical Chemistry
|December 1, 2025
Summary
Accurate measurement of daptomycin (DAP), an important antibiotic, is crucial for patient care and drug development. This review details various analytical methods for quantifying DAP in biological and pharmaceutical samples.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Clinical Science
Background:
- Daptomycin (DAP) is a vital cyclic lipopeptide antibiotic for treating severe Gram-positive bacterial infections.
- Accurate quantification of DAP is essential for therapeutic drug monitoring, pharmacokinetic studies, and quality control in clinical and pharmaceutical settings.
Purpose of the Study:
- To provide a comprehensive review of analytical methods for daptomycin determination.
- To systematically discuss sample preparation strategies for biological matrices.
- To guide researchers in selecting appropriate DAP quantification methods.
Main Methods:
- Literature search of Web of Science, PubMed, and ScienceDirect (2008-2025).
- Review of fifty-one studies on biological matrices (plasma, serum, urine, etc.) and eight on pharmaceutical formulations.
- Analysis of methods including liquid chromatography (LC-UV, LC-MS), spectrofluorimetry, spectrophotometry, voltammetry, and capillary electrophoresis.
Main Results:
- Various analytical techniques have been developed for DAP quantification.
- Effective sample preparation methods like protein precipitation and solid-phase extraction are discussed for biological samples.
- A comparative perspective on experimental evidence for method selection is provided.
Conclusions:
- This review offers a structured overview of analytical methods for DAP determination.
- It highlights the importance of reliable quantification for clinical and pharmaceutical applications.
- It serves as a guide for selecting optimal DAP quantification strategies.
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