Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

998
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
998
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.3K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.3K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.4K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.4K
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

2.7K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
2.7K
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

1.7K
Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Core to Front: Microplastic Loads Along a Signal Crayfish (Pacifastacus leniusculus) Invasion Gradient.

Environmental toxicology and chemistry·2026
Same author

Design and fabrication of plasmonic hedgehog-shaped covalent organic framework nanocomposites for indirect SERS-based ultradetection of water contaminant terbutryn.

Nanoscale·2026
Same author

Characterization of Antidepressant Consumption in a Portuguese Inland Population.

Healthcare (Basel, Switzerland)·2025
Same author

Chromatographic determination of antidepressants in plasma and saliva: Towards non-invasive therapeutic monitoring.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2025
Same author

Natural Slab Photonic Crystals as Biogenic, Customizable Nanomaterial for Label-Free Detection.

ACS applied nano materials·2025
Same author

Diving into the metabolic interactions of titanium dioxide nanoparticles in "Sparus aurata" and "Ruditapes philippinarum".

Environmental pollution (Barking, Essex : 1987)·2024

Related Experiment Video

Updated: Jan 9, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.5K

Automated Background Noise Removal from Substrate Materials in Raman Spectra Using Dynamic Time Warping.

Ensieh Iranmehr1, Monica Quarato1, Marco Martins1

  • 1International Iberian Nanotechnology Laboratory (INL), Braga 4715-330, Portugal.

ACS Omega
|December 1, 2025
PubMed
Summary

This study presents an automated method to remove background noise in Raman spectroscopy, improving data accuracy. The technique effectively subtracts interfering signals, enhancing spectral analysis for various applications.

More Related Videos

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:46

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

16
Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research
08:12

Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research

Published on: February 16, 2024

15.0K

Related Experiment Videos

Last Updated: Jan 9, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.5K
A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:46

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

16
Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research
08:12

Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research

Published on: February 16, 2024

15.0K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy

Background:

  • Raman spectroscopy is vital for point-of-care testing and environmental monitoring.
  • Background signals from matrix interference or substrates hinder accurate spectral interpretation.

Purpose of the Study:

  • To introduce an automated methodology for refining Raman spectra clarity.
  • To address the challenge of background signal interference in Raman analysis.

Main Methods:

  • Developed an automated approach for systematic background subtraction.
  • Implemented a method involving feature extraction from background spectra and subsequent removal.

Main Results:

  • Successfully tested the methodology using simulated and experimental data.
  • Demonstrated the robustness and reliability of the automated background subtraction technique.

Conclusions:

  • The automated method significantly enhances Raman spectral clarity.
  • This technique offers a transformative tool for improving Raman spectroscopic analysis.