Effects of heat stimulation after high-intensity interval training on serum adipokines, eHSP72, and immunoglobulins in men with obesity

Excessive body fat causes imbalances in adipokines and impairs immune function, and not only exercise but also heat stimulation can promote fat reduction and induce extracellular heat shock protein 72 (eHSP72) expression, thereby improving immune function. Therefore, we examined the effects of heat stimulation after HIIT on adipokines, eHSP72, and immunoglobulin levels in men with obesity.

Forty-eight men were randomly assigned to one of the following three groups: high-intensity interval training followed by heat stimulation (HIITHS), moderate-intensity continuous training followed by heat stimulation (MICTHS), and heat stimulation (HS). The 12-week intervention was performed three times per week. Body composition and blood marker levels were assessed before and after the intervention.

The HIITHS and MICTHS groups showed significant improvements in body weight, body mass index (BMI), and body fat percentage. Adiponectin levels increased, whereas leptin levels decreased in all groups. eHSP72 and immune marker levels (IgA, IgG) increased, with the greatest eHSP72 response observed in the HIITHS group. The IgE levels decreased in the HIITHS and HS groups.

These results suggest that combining HIIT with heat stimulation improves serum adipokines, eHSP72, and immunoglobulins in men with obesity, indicating that this approach may serve as a safe and effective intervention.