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Related Experiment Video

Updated: Jan 9, 2026

Two-Dimensional Super-Resolution Visualization of Rat Brain Microvasculature Using Ultrasound Localization Microscopy
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Ultrasound Localization Microscopy for Super-Resolution Mapping of the Rodent Brain Microvasculature.

Pia Virginia Pelaez1, Sara Romanzi1, Adrien Bertolo1

  • 1Iconeus.

Journal of Visualized Experiments : Jove
|December 1, 2025
PubMed
Summary

Ultrasound Localization Microscopy (ULM) provides super-resolution brain imaging by tracking microbubbles, revealing microvascular details. This protocol details rodent ULM for analyzing cerebrovascular changes in disease models.

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Area of Science:

  • Neuroimaging
  • Biomedical Engineering
  • Vascular Biology

Background:

  • Conventional ultrasound is limited by diffraction, hindering visualization of fine brain vasculature.
  • Super-resolution techniques are needed to resolve microvascular architecture in vivo.
  • Ultrasound Localization Microscopy (ULM) offers a solution for high-resolution, label-free vascular imaging.

Purpose of the Study:

  • To present a comprehensive protocol for performing Ultrasound Localization Microscopy (ULM) in rodents.
  • To detail the workflow from animal preparation to data processing for ULM imaging.
  • To enable high-resolution analysis of brain microcirculation in preclinical models.

Main Methods:

  • Utilized a dedicated functional ultrasound platform for ultrafast imaging.
  • Developed distinct preparation methods for mice (transcranial) and rats (cranial windows).
  • Employed microbubble injection and tracking with advanced image reconstruction for super-resolution mapping.

Main Results:

  • Generated high-resolution maps of vascular density, flow velocity, and amplitude (5-10 µm scale).
  • Successfully visualized full coronal planes of rodent brains, identifying microvascular networks.
  • Demonstrated ULM's utility in characterizing cerebrovascular alterations in disease models.

Conclusions:

  • ULM is a powerful, noninvasive tool for preclinical brain microcirculation analysis.
  • The protocol facilitates cross-sectional and longitudinal studies of cerebrovascular diseases.
  • ULM surpasses conventional ultrasound limits for in vivo microvascular imaging.