Related Experiment Video
Updated: Jan 9, 2026
11:13
Porcine Model of Infrarenal Abdominal Aortic Aneurysm
Published on: November 21, 2019
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Experience and Insight Into Genetic Diagnosis of Infective Aortic Aneurysm
Shinnosuke Fukushima1,2,3, Hideharu Hagiya1, Takumi Fujimori4
1Department of General Medicine Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan.
Clinical Case Reports
|December 2, 2025
Summary
Accurate identification of bacteria is vital for treating culture-negative infected aneurysms, especially after antibiotic use. 16S ribosomal RNA gene sequencing offers a precise method for identifying causative bacteria in these challenging cases.
Area of Science:
- Infectious Diseases
- Microbiology
- Vascular Surgery
Background:
- Culture-negative infected aneurysms present diagnostic challenges, often linked to prior antibiotic exposure.
- Accurate microbial diagnosis is essential for effective antimicrobial therapy in these patients.
Purpose of the Study:
- To highlight the importance of precise bacterial identification in culture-negative infected aneurysms.
- To evaluate the utility of 16S ribosomal RNA (rRNA) gene sequence analysis for microbial identification in this context.
Main Methods:
- Review of clinical situations leading to culture-negative infected aneurysms.
- Application of 16S ribosomal RNA gene sequencing for bacterial identification.
Main Results:
- 16S rRNA gene sequencing demonstrated utility in identifying bacteria in culture-negative samples.
- This molecular technique aids in overcoming limitations of traditional culture methods.
Conclusions:
- Precise bacterial identification is critical for optimizing antimicrobial strategies in culture-negative infected aneurysms.
- 16S ribosomal RNA gene sequence analysis is a valuable tool for achieving accurate microbial identification in challenging clinical scenarios.
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