Competitive effect-derived surface-enhanced Raman scattering for analysis of glutathione based on a gold...

Area of Science:

• Nanotechnology
• Biosensing
• Analytical Chemistry

Background:

• Glutathione (GSH) is a crucial antioxidant regulating cellular redox potential and homeostasis.
• Accurate GSH monitoring is vital for understanding physiological conditions and disease progression.
• Existing methods for GSH analysis lack the speed and simplicity required for high-throughput screening.

Purpose of the Study:

• To develop a rapid, efficient, and robust biosensing platform for high-throughput glutathione (GSH) detection.
• To leverage competitive binding interactions for sensitive and selective GSH quantification.
• To integrate nanocapillary technology with SERS for advanced biomolecule analysis.

Main Methods:

• Fabrication of a nanocapillary biosensor via sequential assembly of gold nanotriangles, DNA, Hg2+, gold nanoparticles, and 4-mercaptobenzoic acid.
• Utilizing a competitive binding mechanism involving GSH-Hg2+ coordination and T-Hg2+-T base pairing.
• Employing surface-enhanced Raman scattering (SERS) for quantitative detection of GSH.

Main Results:

• The developed biosensor achieved quantitative GSH detection over a wide concentration range (10^-9 to 10^-3 M).
• A low detection limit of 10^-9 M for GSH was accomplished.
• The platform demonstrated excellent selectivity for GSH against common interfering amino acids.

Conclusions:

• The novel system provides efficient and rapid GSH detection via a competitive mechanism.
• The SERS-integrated nanocapillary platform enables selective and quantitative biomolecule analysis.
• This technology offers a versatile foundation for future nanofluidic devices and biomolecule monitoring.