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Updated: Jan 9, 2026

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
Published on: August 15, 2025
Two high-quality genomes of Prototheca bovis strain SH08 and Prototheca ciferrii strain SH13
Jian Guo1, Yao Ming2, Juan Chen1
1Department of Laboratory Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, 200120, China.
Abstract:
Prototheca, one of the foremost causative agents responsible for opportunistic infections in both humans and animals. Despite the availability of several Prototheca genomes, the genomes of Prototheca bovis and Prototheca ciferrii have remained unsequenced. In this study, we report two high-quality genome assemblies for P. bovis strain SH08 and P. ciferrii strain SH13, representing two distinct species of the genus Prototheca. The final assembled genome sizes were 30.6 Mb for P. bovis SH08 and 32.7 Mb for P. ciferrii SH13, with contig N50 values of 1.19 Mb and 1.78 Mb, respectively. The repetitive sequences were identified in 14.79% and 14.24% of the assembled genomes, respectively. A total of 5,141 protein-coding genes were predicted for P. bovis SH08, while P. ciferrii SH13 contained 4,986 protein-coding genes. Functional annotation identified 97.28% and 99.16% of the genes in the genomes, respectively. These two novel high-quality genome assemblies represent valuable resources for advancing research in evolutionary biology, comparative genomics, pathogenicity assessment, diagnostic strategies, and therapeutic interventions in protothecosis.
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