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Updated: Jun 15, 2026

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

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Published on: December 24, 2010

Biomarker-Gated Photocatalysis Uncaging in Live System.

Guoming Tong¹, Changfeng Li¹, Qing Zhou¹

¹Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research of Ministry of Education, Key Laboratory of Phytochemical R&D of Hunan Province, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, Hunan, 410081, China.

Angewandte Chemie (International Ed. in English)

|December 3, 2025

Summary

This study introduces a novel platform for precise control of biochemical reactions using light. It enables targeted drug delivery and enhanced bioimaging by linking catalyst activity to specific biomarkers and light exposure.

Keywords:

Biomarker‐gated Biosensing Dual‐lock Photocatalysis Prodrug

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Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions

Published on: October 5, 2019

Area of Science:

Biochemistry
Organic Chemistry
Bioimaging
Therapeutics

Background:

Spatiotemporal control of photocontrolled biochemical reactions is a significant challenge.
Existing methods lack precision in triggering reactions and activating therapeutic agents.
Bioorthogonal chemistry and photocatalysis offer potential for spatiotemporal control.

Purpose of the Study:

To develop a sequential activation platform for photocontrolled biochemical reactions.
To enhance bioimaging capabilities through signal amplification.
To enable AND-gate logic for targeted therapeutic activation.

Main Methods:

Engineered diiodo-BODIPY carboxylic acid as an organic photocatalyst.
Masked photocatalyst activity using an ester linkage, restored by enzyme- or ROS-cleavable linkers.
Integrated biomarker-triggered unlocking with photocatalytic bioorthogonal uncaging.

Main Results:

Demonstrated a "lock-and-key" mechanism for photocatalyst activation.
Achieved signal amplification for enhanced bioimaging.
Enabled "AND-gate" therapeutic activation requiring both biomarker presence and light exposure.
Successfully released caged therapeutic agents like RNase A and doxorubicin.

Conclusions:

The developed platform integrates bioresponsive chemistry with light-mediated control.
Offers a blueprint for logical gate-regulated cellular processes.
Advances precision medicine with potential for targeted and efficient therapies.