Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Channel Rhodopsins

Channel Rhodopsins

Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Polymer Chemistry and the Kinetics of Nanoprecipitation.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Molecular Recognition-Driven Reaction-Based Sensing of Catecholamines in a Lipid Nanoreactor.

Journal of the American Chemical Society·2026

Same author

<i><b>De Novo</b></i> Development of Fluorogenic RNA-Based Biosensors Using Capture-SELEX in Tandem with Droplet Microfluidics.

ACS sensors·2025

Same author

Fluorescent Polymeric Nanofibers as Ratiometric Multiplexed Skin Sensors of pH and Oxygen.

Advanced healthcare materials·2025

Same author

Deciphering the Blinking Mechanisms of Dye-Loaded Organic Nanoparticles.

The journal of physical chemistry letters·2025

Same author

Extending the Potential of Digital Droplet PCR by Expressing Amplified Genes and Assaying Their Phenotype.

Methods in molecular biology (Clifton, N.J.)·2025

Same journal

On-Cell Detection of Polysaccharide One-Bond <sup>1</sup>J<sub>CH</sub> Couplings by Proton-Detected Solid-State NMR.

Journal of the American Chemical Society·2026

Same journal

Correction to "Unraveling the Effects of Fe Incorporation on High-Performance Water-Splitting Photoanodes".

Journal of the American Chemical Society·2026

Same journal

Proximity-Driven Protein Ligation Beyond the Concentration Limit.

Journal of the American Chemical Society·2026

Same journal

GraPhAI: Neural Networks for Solving Centrosymmetric Crystal Structures.

Journal of the American Chemical Society·2026

Same journal

Probing Stage Transition Kinetics in Li-Graphite Intercalation Compounds by Time-Resolved In Situ Solid-State NMR via <sup>13</sup>C Labeling.

Journal of the American Chemical Society·2026

Same journal

Dynamic Covalent Programming at DNA Base-Pairing Interfaces.

Journal of the American Chemical Society·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 3, 2026

Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

oShark: A Modified Nuclease-Resistant Light-Up Aptamer.

Janine Kehrli¹, Roger Cubi¹, Tanushree Gupta²

¹Université de Strasbourg, CNRS, Architecture et Réactivité de l'ARN, UPR 9002, F-67000 Strasbourg, France.

Journal of the American Chemical Society

|December 3, 2025

Summary

This summary is machine-generated.

Researchers developed oShark, a modified light-up RNA aptamer, enhancing stability in biological environments. This engineered aptamer maintains function and fluorescence, paving the way for advanced molecular detection tools.

More Related Videos

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Related Experiment Videos

Last Updated: May 3, 2026

Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Area of Science:

Biochemistry
Molecular Biology
RNA Aptamer Technology

Background:

Light-up RNA aptamers offer selective binding and fluorescence activation for molecular detection.
RNA backbone instability in extracellular environments limits aptamer applications.
Chemical modifications can improve stability but may hinder aptamer folding and function.

Purpose of the Study:

To develop a chemically modified light-up RNA aptamer with enhanced stability for extracellular applications.
To maintain aptamer integrity, folding, and fluorogen activation despite chemical modifications.
To create a novel dual light-up aptamer system.

Main Methods:

Directed evolution strategy.
Microfluidic-assisted screening.
Rational design and sequence optimization.

Main Results:

Developed oShark, a fluorinated light-up aptamer activating the Gemini-552 dye.
oShark demonstrated preserved integrity and function in challenging extracellular media.
Engineered Lemon-oShark, the first modified dual light-up aptamer, by fusing oShark with Mango-II.

Conclusions:

oShark represents a significant advancement in stabilizing RNA aptamers for biological applications.
The developed aptamers maintain high fluorescence and brightness comparable to unmodified versions.
This work enables new possibilities for in vivo and in vitro molecular sensing using robust RNA aptamers.