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Microbial Biosensors01:17

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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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De Novo Development of Fluorogenic RNA-Based Biosensors Using Capture-SELEX in Tandem with Droplet Microfluidics.

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Developing new sensor aptamers for fluorogenic RNA-based biosensors (FRBs) is accelerated by combining Capture-SELEX with microfluidic technology. This pipeline efficiently reprograms aptamer specificity for diverse biotechnological applications.

Keywords:
aptamerbiosensordroplet microfluidicsfluorogenic light-up aptamerscreening

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Detection of small molecules like pesticides and pollutants is crucial for health and environmental monitoring.
  • Fluorogenic RNA-based biosensors (FRBs) offer on-site sensing capabilities but require efficient aptamer development.
  • Current methods for developing new sensor aptamers for FRBs are time-consuming.

Purpose of the Study:

  • To accelerate the de novo development of sensor aptamers for FRBs.
  • To reprogram the specificity of existing sensor aptamers for new targets.
  • To demonstrate the versatility of the developed aptamers in biotechnological tools.

Main Methods:

  • Utilized Capture-SELEX in conjunction with microfluidic-assisted in vitro selection (μIVC).
  • Employed fluorescence-based functional selection for screening aptamers.
  • Reprogrammed specificity of existing sensor aptamers.

Main Results:

  • Successfully accelerated the development of novel sensor aptamers for FRBs.
  • Demonstrated the reprogramming of aptamer specificity using the combined technology.
  • Showcased the repurposing of new sensor aptamers into gene-regulating aptazymes.

Conclusions:

  • The Capture-SELEX and μIVC pipeline significantly speeds up sensor aptamer development for FRBs.
  • The developed sensor aptamers are versatile and can be adapted for various biotechnological applications, including aptazymes.
  • This approach provides a robust platform for creating tailored aptamer-based biosensors.