Controlled-environment platform for electrophysiology recordings on organoids with Neuropixels 2.0
Zongren Zhang1, Tjitse van der Molen2,3, Carolina Maciel Camargo2,3
1Department of Physics, University of California, Santa Barbara, Santa Barbara, California 93106, USA.
The Review of Scientific Instruments
|December 3, 2025
Abstract
None:
We present a custom recording platform that enables electrophysiological recordings with Neuropixels 2.0 from human brain and heart organoids by maintaining a stable, physiological environment of 5% CO2, 37 °C, and 95% relative humidity. The platform combines a modular positioning system with real-time camera guidance for precise probe alignment. We validated the system by recording consistent bursting activity in brain organoids over a 110-min period and in heart organoids for up to 11 h.
