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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

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Bioengineered Fluorescent Sensors for Dynamic Oxygen and Lactate Monitoring in Cellular Microenvironments.

Giuliana Grasso, Stefania Forciniti, Valentina Onesto

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference

|December 3, 2025

Summary

Researchers developed novel fluorescent tools to detect oxygen and lactic acid in cell models. These bioengineered systems offer effective quantitative detection for studying cellular microenvironments and diseases like cancer.

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Area of Science:

Biomedical Engineering
Cell Biology
Cancer Research

Background:

Cellular microenvironment dynamics are crucial for physiological processes and disease progression.
Cancer presents a significant global health challenge, necessitating advanced monitoring tools.
Bioengineered systems offer sophisticated methods for studying live cells and their interactions.

Purpose of the Study:

To develop innovative ratiometric fluorescent tools for sensing key cellular metabolites.
To investigate the sensing performance of oxygen-sensing fibers and lactate-sensing microparticles.
To enable quantitative detection of oxygen and lactic acid in cellular models.

Main Methods:

Development of oxygen-sensing fibers and lactate-sensing microparticles.
Comprehensive morphological and physicochemical characterization of the sensing platforms.
Investigation of sensing performance in in vitro models.

Main Results:

Successful creation of two novel ratiometric fluorescent sensing tools.
Demonstrated effective quantitative detection of oxygen and lactic acid.
Validated the tools in in vitro models of healthy and melanoma cells.

Conclusions:

The developed sensing systems are simple and effective for metabolite detection.
These tools aid in understanding cellular microenvironments in health and disease.
The platforms show potential for advancing cancer research and diagnostics.