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Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Related Experiment Video

Updated: Jan 9, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

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Published on: September 5, 2019

In Vivo Positron Emission Particle Tracking (PEPT) of Single Cells Using an Expectation Maximization Algorithm.

Hieu T M Nguyen, Neeladrisingha Das, Rohollah Nasiri

IEEE Transactions on Medical Imaging

|December 3, 2025

Summary

This summary is machine-generated.

This study shows that the PEPT-EM algorithm can track multiple cells simultaneously in vivo. This breakthrough in positron emission particle tracking (PEPT) enables high-throughput cell migration studies.

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Last Updated: Jan 9, 2026

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Published on: September 5, 2019

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Tracking Mouse Bone Marrow Monocytes In Vivo

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Published on: February 27, 2015

Area of Science:

Biomedical Engineering
Cell Biology
Medical Imaging

Background:

Cell tracking is vital for understanding migration in health and disease.
Positron emission particle tracking (PEPT) localizes moving particles non-invasively.
Current PEPT methods are limited to tracking a single cell at a time.

Purpose of the Study:

To investigate the feasibility of simultaneously tracking multiple cells using the PEPT-EM algorithm.
To address the challenge of low radioactivity in cells for biomedical applications.

Main Methods:

Utilized a preclinical PET scanner and the PEPT-EM algorithm.
Experimentally validated performance with droplets and cells (tens to hundreds of Bq).
Tested in phantoms and a murine model to assess dynamic tracking of moving sources.

Main Results:

Successfully localized multiple single cells moving up to 25 mm/s in a murine model.
Demonstrated the critical role of sensitivity for dynamic tracking over background effects.
Marked the first application of PEPT-EM for multi-cell tracking in vivo.

Conclusions:

PEPT-EM is feasible for real-time, high-throughput tracking of multiple single cells in vivo.
This advancement opens new avenues for studying cell migration in biological systems.
PEPT offers significant potential for future in vivo cell tracking applications.