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Related Experiment Video

Updated: Jan 9, 2026

Potato Virus X-Based microRNA Silencing VbMS In Potato.

Potato Virus X-Based microRNA Silencing VbMS In Potato.

Published on: May 11, 2020

A robust absolute quantification protocol for potato virus Y based on reverse transcription-quantitative PCR.

Diana Spencer¹, Elizabeth Padilla-Crespo², Ek Han Tan¹

¹School of Biology and Ecology, University of Maine, Orono, Maine, United States.

Micropublication Biology

|December 4, 2025

Summary

A new standardized protocol accurately quantifies potato virus Y (PVY) in potato tissues. This method uses cloned viral sequences for reliable detection and management of PVY, crucial for host-virus studies.

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Area of Science:

Plant Virology
Molecular Biology
Agricultural Science

Background:

Accurate quantification of potato virus Y (PVY) is essential for understanding host-virus dynamics and managing potato crops.
Existing methods may lack standardization, impacting the reliability of viral load assessments.

Purpose of the Study:

To develop and validate a standardized protocol for the absolute quantification of PVY in potato tissue.
To establish a reliable method for detecting and quantifying PVY across different strains.

Main Methods:

Utilized a cloned viral sequence as an internal standard for quantitative detection.
Employed a column-based kit for total RNA extraction and concentration from potato tissue.
Validated the protocol for linearity, repeatability, reproducibility, and statistical robustness.

Main Results:

The developed protocol can detect approximately 50 viral copies per reaction.
Demonstrated high linearity across 8 orders of magnitude.
Showcased excellent repeatability, reproducibility, and statistical robustness over three independent runs.

Conclusions:

The standardized protocol provides reliable absolute quantification of PVY.
This method supports effective potato crop health management and comparative viral studies.
Enables robust host-virus dynamic research through precise viral load determination.