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Area of Science:

  • Geochemistry
  • Isotope Ratio Mass Spectrometry
  • Metrology

Background:

  • The IAEA endorsed two δ13C scales: Vienna Peedee belemnite (VPDB) and VPDB-LSVEC.
  • Instability of the LSVEC material necessitated the search for replacement reference materials (RMs).
  • This study addresses the need for reliable RMs to maintain metrological traceability for δ13C measurements.

Purpose of the Study:

  • To compare proposed calcium carbonate RMs (IAEA-610, -611, -612, USGS44) for the VPDB and VPDB-LSVEC δ13C scales.
  • To recommend values for these RMs to enable users to realize the VPDB and VPDB-LSVEC scales accurately.
  • To scrutinize the phosphoric acid reaction method for CO2 evolution in isotope analysis.

Main Methods:

  • Comparison of four calcium carbonate RMs (IAEA-610, -611, -612, USGS44) across three expert laboratories.
  • Utilization of high-precision dual-inlet isotope ratio mass spectrometers.
  • Assessment of instrument offsets and interlaboratory comparability of samples.

Main Results:

  • Reported δ13CVPDB values for IAEA-610, -611, -612, and USGS44 are -9.114 ± 0.011‰, -30.815 ± 0.011‰, -36.739 ± 0.020‰, and -42.073 ± 0.015‰, respectively.
  • These values are consistent with previously published data within measurement uncertainty.
  • Values on both δ13CVPDB and δ13CVPDB-LSVEC scales are provided for routinely used RMs in elemental analysis-isotope ratio mass spectrometry.

Conclusions:

  • The study successfully compared and validated new calcium carbonate RMs for the VPDB and VPDB-LSVEC δ13C scales.
  • Recommended values enable accurate realization of these critical isotopic scales.
  • Findings support the continued accuracy and comparability of δ13C measurements in scientific research.