Related Experiment Video
Updated: Aug 4, 2026
Rapid High Throughput Amylose Determination in Freeze Dried Potato Tuber Samples
Published on: October 14, 2013
Comparison of three different calibration methods and quadruple isotope dilution strategy to design more accurate
Süleyman Bodur1, Sezin Erarpat Bodur2, Beste Coşar2
1Yıldız Technical University, Faculty of Art and Science, Department of Chemistry, 34220 İstanbul, Türkiye; İstinye University, Faculty of Pharmacy, Department of Analytical Chemistry, 34010 İstanbul, Türkiye; İstinye University, Scientific and Technological Research Application and Research Center, 34010 İstanbul, Türkiye.
Abstract:
A highly accurate and precise quadruple isotope dilution (ID4) strategy was employed for the determination of monuron in potato flour samples. Isotopic compositions were monitored by tandem mass spectrometry (MS/MS). Isotopically labelled monuron substance (monuron-d6) was synthesized in our research laboratory. After optimizing HPLC-MS/MS system, limit of detection (LOD), limit of quantitation (LOQ) and linear working range (LWR) were figured out as 25.28 ng/kg, 84.27 ng/kg and 0.08-27.55 μg/kg, respectively. ID4 method was carried out by spiked potato flour samples. Percent recovery results (%RRs) for HPLC-ID4-MS/MS system were varied from 99.0 % to 101.7 % with low standard deviation values (1.2-2.3 %). HPLC-ID4-MS/MS method gave excellent accuracy and precision to determine monuron at trace levels in potato flour samples when compared to external, internal and matrix matching calibration strategies. According to the current literature, no study has been employed to determine monuron in potato flour samples by the HPLC-ID4-MS/MS system.
