Candidate Prognostic LncRNAs Including C2orf49-DT, CAPN10-DT, LOC105371795 as Potential Biomarkers for Colorectal...

Area of Science:

• Molecular Biology
• Genetics
• Oncology

Background:

• Long non-coding RNAs (lncRNAs) play regulatory roles in cellular processes and are implicated in various diseases, including cancer.
• Identifying specific lncRNAs associated with colorectal cancer (CRC) pathogenesis is crucial for developing novel diagnostic and prognostic biomarkers.

Purpose of the Study:

• To investigate signature lncRNAs associated with colorectal cancer (CRC) pathogenesis.
• To evaluate the potential of identified lncRNAs as diagnostic and prognostic biomarkers for CRC.

Main Methods:

• Utilized The Cancer Genome Atlas (TCGA) data to analyze lncRNA expression and its correlation with patient mortality.
• Employed linear models for differential expression analysis and Cox regression for prognostic association.
• Validated in-silico findings using qRT-PCR on 35 ex vivo CRC tumor samples.

Main Results:

• Identified C2orf49-DT, CAPN10-DT, and LOC105371795 as significantly upregulated in CRC samples compared to healthy tissues.
• Demonstrated a correlation between elevated expression of these lncRNAs and poorer patient prognoses.
• Co-expression network analysis revealed associations with cell proliferation pathways, including E2F3.

Conclusions:

• Elevated levels of C2orf49-DT, CAPN10-DT, and LOC105371795 are associated with increased CRC mortality.
• These lncRNAs, linked to cell proliferation pathways, show promise as potential biomarkers for CRC diagnosis and prognosis.