Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fats as Energy Storage Molecules01:06

Fats as Energy Storage Molecules

26.8K
Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis...
26.8K
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.7K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.7K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.2K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.2K
Coupled Reactions01:17

Coupled Reactions

10.5K
Cellular processes such as building and breaking down complex molecules occur through stepwise chemical reactions. Some of these chemical reactions are spontaneous and release energy, whereas others require energy to proceed. Cells often couple the energy-releasing reaction with the energy-requiring one to carry out important cell functions. 
Energy in adenosine triphosphate or ATP molecules is easily accessible to do work. ATP powers the majority of energy-requiring cellular reactions....
10.5K
The ADP/ATP Carrier Protein01:42

The ADP/ATP Carrier Protein

4.1K
ADP/ATP carrier or AAC protein is the most abundant carrier protein in the inner mitochondrial membrane. It transports large quantities of ADP and ATP, equivalent to the average human body weight, every day. Among other transporters, ACC protein is one of the best-studied members of the mitochondrial carrier protein family. The ADP/ATP carrier protein comprises two transmembrane helices connected to a loop and a single alpha-helix on the matrix side. It switches between two conformational...
4.1K
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

784
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
784

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Otopetrin 1 protects against adipose tissue wasting during cancer cachexia progression.

iScience·2026
Same author

The Sex-Stratified Effects of RSPO3 Expression on Body Fat and Blood Lipids: An Omics Mendelian Randomization Study.

Obesity (Silver Spring, Md.)·2026
Same author

Evolutionary tuning of a biosynthetic gene cluster drives furanocoumarin accumulation and diversification.

Nature communications·2026
Same author

Sex-Specific Outcomes and Treatment Responses in Elderly Patients With Heart Failure With Preserved Ejection Fraction: A Comprehensive Single-Center Retrospective Study.

Journal of the American Heart Association·2026
Same author

The 1-hour plasma glucose as a specific marker for early-phase insulin secretory defects in young adults with obesity.

Diabetes research and clinical practice·2026
Same author

Paeonol mitigates age-related osteoporosis via mitophagy-mediated NLRP3 inflammasome inhibition.

International immunopharmacology·2026

Related Experiment Video

Updated: Jan 9, 2026

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.8K

Uncoupling protein 1 in nonadipose tissue.

Xi Hu1,2, Donghua Hu1,2, Kun Chen1

  • 1Department of Cardiology, Huadong Hospital, Fudan University, Shanghai, People's Republic of China.

American Journal of Physiology. Endocrinology and Metabolism
|December 5, 2025
PubMed
Summary

Uncoupling protein 1 (UCP1) is expressed in various embryonic non-adipose tissues, not just for thermogenesis. UCP1 deficiency causes retinal defects, indicating broader physiological roles beyond heat production.

Keywords:
Ucp1-promoter-driven Creembryonic developmentgene expressionnonadipose tissueuncoupling protein 1

More Related Videos

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
07:03

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Published on: June 12, 2020

10.4K
Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue
06:28

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Published on: October 7, 2014

13.8K

Related Experiment Videos

Last Updated: Jan 9, 2026

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.8K
Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
07:03

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Published on: June 12, 2020

10.4K
Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue
06:28

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Published on: October 7, 2014

13.8K

Area of Science:

  • Mitochondrial Biology
  • Developmental Biology
  • Genetics

Background:

  • Uncoupling protein 1 (UCP1) is traditionally known as a mitochondrial protein in thermogenic adipocytes.
  • The use of UCP1-promoter-driven Cre for gene manipulation in thermogenic adipocytes is common, but its activity in non-adipocyte types is increasingly recognized.
  • The expression patterns and non-thermogenic functions of UCP1 in non-adipose tissues during development are not well understood.

Purpose of the Study:

  • To systematically investigate UCP1 expression patterns from embryogenesis to adulthood in various tissues.
  • To explore the potential non-thermogenic physiological roles of UCP1 in non-adipose tissues.
  • To compare the efficacy of different Cre strategies for gene manipulation in thermogenic adipocytes.

Main Methods:

  • Utilized UCP1-GFP, UCP1-CreERT2 knock-in, and UCP1-Cre transgenic mouse models crossed with Ai9-tdTomato-Red reporter mice.
  • Employed single-cell RNA sequencing and immunostaining analyses to determine UCP1 expression.
  • Generated UCP1-CreERT2/CreERT2 knockout mice to assess developmental consequences of UCP1 deficiency.

Main Results:

  • UCP1 expression was detected in non-thermogenic tissues, including the brain, eye, ear, mammary gland, kidney, and reproductive systems, starting as early as embryonic day 10.5.
  • UCP1 expression was more widespread in non-adipose tissues during embryonic development than in adulthood, particularly in epithelial cells.
  • UCP1 knockout mice displayed retinal developmental defects, suggesting roles beyond thermogenesis.

Conclusions:

  • UCP1 has significant physiological functions in non-adipose tissues during development, extending beyond its role in thermogenesis.
  • The study provides evidence for UCP1's involvement in retinal development.
  • Ucp1-promoter-driven tamoxifen-inducible Cre offers a more precise tool for gene manipulation in thermogenic adipocytes, minimizing off-target effects compared to traditional transgenic Cre methods.