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Conditional deletion of Tmem63b does not impact mouse voiding behavior.

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Area of Science:

  • Urology
  • Cell Biology
  • Neuroscience

Background:

  • Lower urinary tract function relies on mechanosensation, but involved sensors are poorly understood.
  • TMEM63 ion channels are known mechanosensors in other tissues.
  • Expression of Tmem63a/b and TMEM63B protein was investigated in the mouse bladder.

Purpose of the Study:

  • To investigate the role of TMEM63 ion channels in lower urinary tract function.
  • To determine if Tmem63b deletion in urothelium or sensory neurons affects voiding behavior.

Main Methods:

  • Generated conditional knockout mice for Tmem63b in urothelial and sensory neurons.
  • Assessed voiding behavior using video-monitored void-spot screening.
  • Utilized cyclophosphamide to stress the urothelium.

Main Results:

  • Tmem63a, Tmem63b, and TMEM63B were expressed in the mouse bladder urothelium and dorsal root ganglia sensory neurons.
  • Conditional urothelial Tmem63b knockout mice showed no significant difference in voiding behavior.
  • Conditional sensory neuron Tmem63b knockout mice also exhibited normal voiding behavior, even after cyclophosphamide treatment.

Conclusions:

  • The urothelium and dorsal root ganglia are sites of Tmem63a/b and TMEM63B expression.
  • Tmem63b deletion in these specific tissues does not result in a detectable voiding phenotype.
  • The role of TMEM63 channels in bladder mechanosensation requires further investigation.