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Extracellular Matrix-Coated Vesicles as a Biomimetic Model of MembraneMatrix Interplay.

Keel Yong Lee1,2,3, Huong Thanh Nguyen1, Sungwoo Jeong1

  • 1Department of Chemistry and Institute of Biological Interfaces, Sogang University, Seoul, 04107, Republic of Korea.

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|December 6, 2025
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Summary

This study introduces a biomimetic platform to explore how extracellular matrix (ECM) proteins like fibronectin and collagen affect cell membrane properties, revealing protein-specific impacts on vesicle mechanics and lipid dynamics.

Keywords:
collagenextracellular matrixfibornectingiant unilamellar vesiclesliposomes

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Area of Science:

  • Biophysics
  • Materials Science
  • Cell Biology

Background:

  • Artificial membrane systems lack the complexity of cellular environments, particularly the influence of the extracellular matrix (ECM).
  • Understanding ECM-membrane interactions is crucial for cell mechanics and extracellular vesicle biology.

Purpose of the Study:

  • To develop a biomimetic platform integrating fibronectin (FN) and collagen type I (COL) onto giant unilamellar vesicles (GUVs).
  • To investigate how ECM coating modulates GUV properties, including curvature, mechanical resilience, and lipid diffusivity.
  • To explore ECM-induced changes in lipid domain organization and vesicle biogenesis.

Main Methods:

  • Coating giant unilamellar vesicles (GUVs) with fibronectin (FN) and collagen type I (COL).
  • Analyzing ECM-specific effects on vesicle curvature, mechanical properties, and lipid diffusion using advanced imaging techniques.
  • Observing and characterizing vesicle budding and lipid domain behavior.

Main Results:

  • ECM coating significantly alters GUV properties in a protein-dependent manner.
  • Fibronectin (FN) promotes vesicle budding and membrane softening.
  • Collagen type I (COL) induces rugged membrane topographies and mechanical stiffening.
  • ECM proteins reshape lipid domain geometry and stability, mimicking cellular membrane heterogeneity.
  • FN-coated GUVs exhibit budding events resembling exosome release.

Conclusions:

  • The biomimetic platform effectively captures ECM-plasma membrane mechanobiological interactions without transmembrane linkers.
  • ECM identity influences membrane mechanics and may regulate vesicle biogenesis, offering insights into exosome formation.
  • This tunable system advances the study of ECM-membrane coupling and ECM-vesicle interplay for synthetic cell engineering and extracellular vesicle biology.