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Updated: Jan 9, 2026

Design and Synthesis of a Reconfigurable DNA Accordion Rack

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Published on: August 15, 2018

Reversible Sequence-Dependent DNA Coacervation with an Azobenzene Intercalator.

Yunzhe Li¹, Julie Pham¹, Mathieu Morel¹

¹CPCV, UMR8228, Department of Chemistry, PSL University, Sorbonne Université, CNRS, Ecole Normale Supérieure, 75005 Paris, France.

Langmuir : the ACS Journal of Surfaces and Colloids

|December 6, 2025

Summary

This summary is machine-generated.

Researchers introduced sequence sensitivity to DNA coacervates using a novel DNA binder, AzodiGua. This allows for light-controlled assembly and disassembly, expanding applications in biomaterials and therapeutics.

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Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

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Published on: February 6, 2020

Area of Science:

Supramolecular Chemistry
Biomaterials Science
Molecular Engineering

Background:

DNA coacervates are associative assemblies driven by electrostatic interactions and entropic gains.
Current DNA coacervates lack sequence specificity, limiting their applications.
High local DNA concentrations in coacervates offer potential in therapeutics, biomimicry, and biosensing.

Purpose of the Study:

To introduce sequence sensitivity into DNA coacervation.
To develop photosensitive DNA coacervates using an azobenzene-based DNA binder.
To explore tunable control over DNA coacervate formation and dissolution.

Main Methods:

Utilized an azobenzene-based DNA binder, AzodiGua, for intercalation into DNA base pairs.
Investigated the effect of AzodiGua concentration on coacervation for different DNA structures (double-stranded vs. single-stranded) and GC content.
Explored photoswitching of coacervates via trans/cis isomerization of the azobenzene moiety upon UV and blue light illumination.
Investigated reversed-photocontrol using alpha-cyclodextrin to sequester trans-AzodiGua.

Main Results:

AzodiGua successfully imparted sequence sensitivity to DNA coacervation.
Coacervation efficiency varied with DNA structure and GC base pair fraction/distribution.
DNA coacervates exhibited reversible photosensitivity, dissolving under UV light and reforming with blue light.
Selective sequestration of trans-AzodiGua by alpha-cyclodextrin enabled a reversed-photocontrol regime.

Conclusions:

AzodiGua enables sequence-specific and photosensitive DNA coacervates.
Light-triggered control over coacervate formation and dissolution is achievable.
The developed system offers novel strategies for advanced biomaterials and drug delivery systems.