Protocol for the measurement and analysis of human photoencephalographic signals

Photoencephalography, involving the measurement of ultraweak photon emissions (UPEs), is increasingly being recognized for its potential significance in understanding brain activity. We present a protocol for recording, analyzing, and interpreting UPE signals in humans. We outline the steps for simultaneous measurement of UPEs and electric fields around the head, using photomultiplier tubes and electroencephalography, as well as data processing and interpretation. This protocol guides the emerging field of biophotonics, establishing a structured pipeline for UPE acquisition and analysis in humans. For complete details on the use and execution of this protocol, please refer to Casey et al.¹.