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Synergistic effects of magnesium-conditioned chitosan hydrogels and photobiomodulation on MC3T3-E1 preosteoblast
Hao-Hsuan Chiu1, Pei-Chun Wong2, Chen Ying-Chun3
1Department of Mechanical Engineering, National Taipei University of Technology, Taipei, Taiwan.
Background:
Magnesium (Mg) and photobiomodulation (PBM) have independently been reported to promote osteogenesis, but their combined effects remain unclear.
Methods:
Mg-conditioned chitosan hygrogels (0,20, and 50 mol%) were prepared, and conditioned media were applied to MC3T3-E1 preosteoblasts with or without near-infrared PBM (830 nm, 5 J/cm²). Cell viability, migration, and calcium deposition were assessed (n = 3 biological replicates per group).
Results:
High Mg reduced cell migration and mineralization, whereas PBM significantly enhanced calcium deposition and partially restored viability at early time points (p < 0.05). A notable synergistic effect was observed at 20 mol% Mg combined with PBM, particularly at specific time points, indicating a condition-dependent rather than uniformly optimal response.
Conclusion:
The combination of Mg-conditioned hydrogels and PBM enhanced osteogenic responses in MC3T3-E1 preosteoblasts. This dual-modality strategy may inform scaffold design in bone tissue engineering.
