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Related Experiment Video

Updated: Jan 9, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

23.1K

Dissecting cellular ecosystem with single-cell CRISPR screens.

Zhirui Liu1,2,3, Zilin Lan1,2,3, Xiaoli Kang2

  • 1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.

Blood Science (Baltimore, Md.)
|December 8, 2025
PubMed
Summary
This summary is machine-generated.

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Single-cell CRISPR screening offers unbiased gene function discovery by profiling transcriptomes and epigenomes. Advances in these powerful functional genomics tools enhance precision and scalability for translational research.

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Traditional CRISPR screens rely on cell fitness or markers, limiting unbiased discovery.
  • Single-cell CRISPR screening integrates pooled screens with omics for comprehensive genetic manipulation profiling.
  • Numerous single-cell CRISPR platforms have been developed over the last decade.

Purpose of the Study:

  • To summarize recent advances in single-cell CRISPR technologies.
  • To outline the applications of these innovative screening platforms.
  • To provide a comparative framework for selecting appropriate platforms.

Main Methods:

  • Combining pooled CRISPR screens with single-cell omics platforms (e.g., transcriptomics, epigenomics).
  • Iterative protocol refinements to improve precision, scalability, and reproducibility.
Keywords:
Functional genomicsSingle-cell CRISPR screen

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Last Updated: Jan 9, 2026

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  • Comparative analysis of various single-cell CRISPR platforms including Perturb-seq, CROP-seq, ECCITE-seq, Direct-seq, and Mosaic-seq.

    • Main Results:

    • Single-cell CRISPR technologies enable comprehensive profiling of transcriptomes and epigenomes after genetic manipulation.
    • Protocol advancements have significantly improved the precision, scalability, and reproducibility of functional genomics studies.
    • Emerging platforms are incorporating spatial resolution, multi-omics, and AI for enhanced interpretation.

    Conclusions:

    • Single-cell CRISPR screening is revolutionizing biological discovery and functional genomics.
    • Despite persistent technical challenges, ongoing innovation is driving the field forward.
    • Future platforms promise to bridge genetic perturbation insights with system-level biological understanding.