Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

6.4K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.4K
Selectins01:25

Selectins

4.1K
Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
4.1K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.7K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.7K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.6K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.6K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

10.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.4K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

9.8K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Auranofin Suppresses Cancer Cell Invasion by Inhibiting Heparanase-1 Expression via the aPKC-NF-κB Pathway.

International journal of molecular sciences·2026
Same author

Exploring the GM-CSF histidine triad as a modulator of structure, molecular motion, and ligand binding.

RSC chemical biology·2026
Same author

Probing Glycosaminoglycan-Protein Interactions: Applications of Surface Plasmon Resonance.

Biosensors·2026
Same author

Marine Derived Natural Products: Emerging Therapeutics Against Herpes Simplex Virus Infection.

Biomolecules·2026
Same author

Novel regulators of heparan sulfate proteoglycans modulate cellular uptake of α-synuclein fibrils.

Communications biology·2025
Same author

Applications of Surface Plasmon Resonance in Heparan Sulfate Interactome Research.

Biomedicines·2025

Related Experiment Video

Updated: Jan 9, 2026

Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation
09:04

Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation

Published on: September 7, 2010

11.9K

N-Sulfated Heparan Sulfate Promotes Reelin Signaling as a Co-receptor.

Lin Pan1,2, Xuehong Song3, Guowei Su4

  • 1Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, United States.

Journal of the American Chemical Society
|December 8, 2025
PubMed
Summary

Heparan sulfate (HS) acts as a coreceptor for Reelin signaling. HS N-sulfation is critical for Reelin binding and receptor activation, offering new therapeutic targets for brain diseases.

More Related Videos

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System
04:47

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Published on: May 22, 2020

3.9K
Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
13:36

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Published on: October 20, 2023

4.1K

Related Experiment Videos

Last Updated: Jan 9, 2026

Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation
09:04

Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation

Published on: September 7, 2010

11.9K
Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System
04:47

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Published on: May 22, 2020

3.9K
Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
13:36

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Published on: October 20, 2023

4.1K

Area of Science:

  • Neuroscience
  • Glycobiology
  • Molecular Biology

Background:

  • Heparan sulfate (HS) is crucial for signal transduction.
  • Reelin is a key signaling protein in brain development and function.
  • A Reelin variant with enhanced HS binding shows resilience to Alzheimer's disease.

Purpose of the Study:

  • To systematically investigate the glycan determinants of Reelin-HS interactions.
  • To elucidate the role of HS in Reelin receptor activation.

Main Methods:

  • Surface plasmon resonance (SPR) to measure Reelin-HS binding affinity.
  • Glycan array analysis to identify critical HS features.
  • Cell surface binding assays and split-luciferase assays to assess Reelin signaling.

Main Results:

  • Full-length Reelin binds HS with high affinity, enhanced by the COLBOS variant.
  • HS N-sulfation is essential for Reelin-HS binding.
  • HS acts as a coreceptor for Reelin-induced ApoER2 dimerization.

Conclusions:

  • Heparan sulfate is established as a coreceptor for Reelin signaling.
  • N-sulfation is a key determinant for Reelin-HS recognition.
  • Findings provide insights into neurodevelopmental/neurodegenerative diseases and suggest HS sulfation as a therapeutic target.