Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioreactor Controls-III01:22

Bioreactor Controls-III

70
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
70
Methods of Medium Optimization01:28

Methods of Medium Optimization

74
Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...
74
Scale-Up Processes01:14

Scale-Up Processes

119
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
119
Upstream Processing01:27

Upstream Processing

102
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
102
Production of Antibiotics01:27

Production of Antibiotics

281
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
281
Production of Pharmaceuticals01:30

Production of Pharmaceuticals

99
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
99

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same authorSame journal

Plasmid design, cloning, and expression of <i>Vitreoscilla</i> hemoglobin in <i>Streptomyces pilosus</i>: a novel strategy to enhance desferrioxamine B production and yield.

Preparative biochemistry & biotechnology·2026
Same author

Production Enhancement of Surface-Expressed D-hydantoinase and D-carbamoylase in E. Coli by Developing a Novel Coculture Process for the Efficient Biotransformation of D-p-hydroxyphenylglycine.

The protein journal·2025
Same author

Effect of culture media and fermentation process on the refolding and purification of rh-GCSF.

Protein expression and purification·2025
Same author

Bioprocess Engineering Strategies for the Overproduction of Surface-Expressed Protein in Escherichia coli: A Review.

Biotechnology and applied biochemistry·2025
Same author

Investigation of the effect of <i>Streptomyces pilosus</i> morphology and effective factors on desferrioxamine B production based on Taguchi experimental design.

Preparative biochemistry & biotechnology·2025
Same author

Expression of <i>Vitreoscilla</i> hemoglobin gene in different organisms: a new approach to increase the efficiency of various biological processes.

Preparative biochemistry & biotechnology·2025

Related Experiment Video

Updated: May 6, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.6K

Enhancing Desferrioxamine B production by Streptomyces pilosus through morphological and process optimization using

Shadi Mosleh Moghadam1, Valiollah Babaeipour1, Rasoul Khalilzadeh1

  • 1Research Center of Science and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran.

Preparative Biochemistry & Biotechnology
|December 8, 2025
PubMed
Summary

This study optimized Desferrioxamine B (DFOB) production by Streptomyces pilosus. Combining medium optimization and bioreactor process control achieved record yields, offering insights for industrial applications.

Keywords:
BioreactorDesferrioxamine BStreptomyces pilosusmorphologyresponse surface methodology (RSM)siderophore production

More Related Videos

High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils
12:36

High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils

Published on: February 9, 2019

13.1K
Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
09:28

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Published on: May 18, 2020

9.2K

Related Experiment Videos

Last Updated: May 6, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.6K
High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils
12:36

High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils

Published on: February 9, 2019

13.1K
Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
09:28

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Published on: May 18, 2020

9.2K

Area of Science:

  • Microbiology
  • Biotechnology
  • Biochemical Engineering

Background:

  • Desferrioxamine B (DFOB) is a vital siderophore with significant therapeutic applications.
  • Optimizing DFOB production by Streptomyces pilosus is crucial for meeting clinical demand.
  • Previous studies have explored various factors influencing DFOB yield, but record-level productivity remains a target.

Purpose of the Study:

  • To enhance Desferrioxamine B (DFOB) yield and productivity from Streptomyces pilosus.
  • To optimize fermentation conditions using statistical methods like Response Surface Methodology (RSM) and the Taguchi method.
  • To investigate the impact of medium composition and process parameters on DFOB biosynthesis.

Main Methods:

  • Response Surface Methodology (RSM) was employed to optimize yeast extract, MgSO4·7H2O concentration, and buffer molarity for flask cultures.
  • The Taguchi method (L9 orthogonal array) was utilized in a 2-L bioreactor to optimize glucose, yeast extract, and agitation speed at a controlled pH.
  • Field Emission Scanning Electron Microscopy (FESEM) was used to analyze microbial morphology under different agitation rates.

Main Results:

  • RSM optimization in flasks yielded 1.55 g/L DFOB (0.63 g/L·day), the highest reported flask productivity.
  • Bioreactor optimization using the Taguchi method achieved 2.35 g/L DFOB (0.235 g/L·h), the highest reported bioreactor productivity.
  • FESEM revealed that excessive agitation led to mycelial fragmentation, negatively impacting DFOB production.

Conclusions:

  • Optimized medium composition and controlled fermentation parameters significantly enhance DFOB production in Streptomyces pilosus.
  • The study achieved unprecedented DFOB yields in both flask and bioreactor systems.
  • Findings provide a foundation for scaling up DFOB production for industrial applications.