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Updated: Apr 16, 2026

A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its Malignancies
Published on: March 8, 2014
A high-throughput bone marrow 3D co-culture system to study resistance to BCR signalling targeted agents in B-NHL
Alex Zadro1,2, Alberto Arribas2,3, Maria Vittoria Colombo1
1Regenerative Medicine Division, Institute for Translational Research, Faculty of Biomedical Sciences, Università Della Svizzera Italiana (USI)-Ente Ospedaliero Cantonale (EOC), Bellinzona, Switzerland.
Abstract:
Bone marrow (BM) involvement in B-cell non-Hodgkin lymphoma (B-NHL) is associated with poor prognosis, as the BM microenvironment provides a protective niche that promotes therapeutic resistance. We developed a simplified, automated and high-throughput 3D BM co-culture model that faithfully reproduces key tumour-stroma interactions. In our system, BM stromal cells (BMSCs) decreased lymphoma cell sensitivity to Phosphatidylinositol 3-kinase (PI3K) and BTK inhibitors. Moreover, we show that our 3D platform enables the mechanistic studies of microenvironment-mediated drug resistance and has the potential to be developed into a tool for personalized therapeutic strategies for B-NHL.
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