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Updated: Jan 9, 2026
12:37
Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
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A QIIME2-based workflow for multi-amplicon 16S rRNA profiling
Armando G Licata1, Marica Zoppi1, Chiara Dossena1
1Integrated Biology of Rare Tumors, Department of Experimental Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
Microbiology Resource Announcements
|December 9, 2025
Abstract
None:
We present an open-source QIIME2 pipeline for 16S multi-amplicon sequencing. Benchmarked against proprietary software with a mock community, our workflow demonstrates comparable sequencing depth and taxonomic accuracy (F1-Score=0.875). The multi-region approach outperforms single amplicons, validating our pipeline as a robust alternative for semiconductor-based sequencing data.
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