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Related Experiment Video

Updated: Jun 26, 2026

An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm

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Published on: April 16, 2018

Multi-Resistant Staphylococcus aureus Growth Inhibition Using an Innovative High Voltage Nanosecond Pulser: In Vitro

Stavros Balasis¹, Konstantinos Papageorgiou², Sophia Georgiou³

¹Department of Orthopedics, University of Patras, Medical School, Patras, Greece.

Microbiologyopen

|December 9, 2025

Summary

This summary is machine-generated.

Novel high-voltage nanosecond electric pulses effectively inhibit the growth of multi-resistant bacteria, specifically Staphylococcus aureus. This innovative approach offers a promising alternative to traditional antibiotics for combating antimicrobial resistance.

Keywords:

electric field pulser electroporation nanosecond radiofrequency pulses staphylococcus aureus

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Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Published on: April 21, 2023

Area of Science:

Biomedical Engineering
Microbiology
Electroporation

Background:

Antimicrobial resistance is a critical global health challenge, necessitating novel therapeutic strategies.
Existing chemotherapeutic agents face expanding bacterial tolerance, driving the need for alternative treatments.
High voltage electric pulses, such as Irreversible Electroporation (IRE), present a promising alternative for bacterial control.

Purpose of the Study:

To describe a new prototype high-voltage nanosecond pulser.
To validate the effectiveness of this device in inhibiting the in-vitro growth of a clinical, antibiotic-resistant Staphylococcus aureus strain.

Main Methods:

A novel high-voltage nanosecond pulser was developed.
Radiofrequency (RF) pulses with varying widths (100 ns, 450 ns) and repetition rates (1 Hz, 1 kHz) were applied.
Therapy durations ranged from 20 to 200 seconds, with electric field strengths up to 11.5 kV/cm.

Main Results:

The nanosecond electric pulsed fields demonstrated significant in-vitro growth inhibition of Staphylococcus aureus.
Increasing electric field strength and therapy duration led to a 3.5-log scale reduction in bacterial cells.
The prototype device successfully inhibited S. aureus growth in in-vitro tests.

Conclusions:

Nanosecond electric pulsed fields represent an effective method for controlling multi-resistant bacteria.
The developed prototype shows potential for treating bacterial infections.
Further ex-vivo studies are recommended to establish a therapeutic protocol for infected skin wounds.