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Related Concept Videos

Membrane Fluidity01:23

Membrane Fluidity

172.1K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
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Membrane Fluidity01:26

Membrane Fluidity

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Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
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Protein Diffusion in the Membrane01:24

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Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
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Updated: Jan 9, 2026

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Biophysical-to-DNA Catalytic Cumulative Birecorder for Measuring Continuous Membrane Fluidity.

Mengying Ye1, Xinyin Li1, Lu Huang1

  • 1Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, P. R. China.

Journal of the American Chemical Society
|December 9, 2025
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Summary
This summary is machine-generated.

Scientists developed a new method to record cell membrane fluidity over time using DNA. This tool, BioDIMER, tracks changes in membrane fluidity during cell cycles, cardiac hypertrophy, and senescence, revealing dynamic cell behaviors.

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Area of Science:

  • Cell Biology
  • Biophysics
  • Molecular Biology

Background:

  • Recording cellular biophysical processes is essential for understanding cell behavior.
  • Developing methods to continuously monitor membrane fluidity has been a significant challenge.

Purpose of the Study:

  • To develop a novel system for recording continuous membrane fluidity events.
  • To enable in situ imaging and digital quantification of membrane dynamics.

Main Methods:

  • Engineered DNAzyme probe sets were used to create a biophysical-to-DNA catalytic cumulative birecorder (BioDIMER).
  • BioDIMER accumulates bimodal signals responsive to transient membrane encounters, recording events in both fluorescent signals and DNA unique molecular identifiers (UMIs).
  • In situ imaging captured spatiotemporal information, while UMI sequencing provided digital quantification of membrane fluidity events.

Main Results:

  • The BioDIMER system successfully recorded continuous membrane fluidity changes in various cell types and states.
  • Differential membrane fluidity dynamics were observed during cell cycle phases, showing an initial decrease followed by an increase.
  • Cardiac hypertrophy was associated with enhanced membrane fluidity, whereas cellular senescence led to a significant reduction.

Conclusions:

  • The BioDIMER method provides a powerful tool for multidimensional interpretation of membrane fluidity timing.
  • The findings highlight the highly dynamic organization of cell membrane components.
  • This approach offers new insights into cell membrane responses to different physiological and pathological states.